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- 详细信息
- 文献和实验
- 技术资料
- 库存:
充足
- 英文名:
Add&Read® Human IFN gamma Quantitative Detection Kit
- 供应商:
南京诺唯赞生物科技股份有限公司
- 保存条件:
-30 ~ -15℃储存,运输条件:≤0℃。
- 规格:
96 tests/500 tests/10000 tests
产品描述
干扰素γ(IFN gamma)是一种主要由T细胞和NK细胞产生的促炎性细胞因子,在体内主要起到病毒防御作用。IFN gamma在细胞内组装,形成可溶二聚体,并分泌到细胞外。IFN gamma参与抗病毒,巨噬细胞活化,增强中性粒细胞和单核细胞功能以及MHC-I和-II在细胞上的表达。
本试剂盒采用夹心法检测IFN gamma含量,试剂盒中有IFN gamma Standard以及两株特异性识别IFN gamma的单克隆抗体,其中一株抗体偶联Eu(供体,Anti-IFN gamma-Eu),另一株抗体偶联A3(受体,Anti-IFN gamma-A3)。当两株抗体同时与IFN gamma结合时,Anti-IFN gamma-Eu和Anti-IFN gamma-A3距离靠近,可发生荧光共振能量转移(FRET)。使用320/340 nm的激发光激发荧光供体,供体发射620 nm的光,此620 nm的光激发荧光受体,受体发射出665 nm的光。样本中待测IFN gamma浓度与FRET信号值(665 nm/620 nm的光强度比值)成正比。
产品特点
均相检测:免洗ELISA,无需包被、洗板,直接加样-孵育-上机检测,节省人力、时间
节约样本:总体系仅20μl
体系稳定:24h内重复检测,信号值基本不变
高通量筛选:可搭载384孔板进行高通量筛选
检测原理

产品组分

储存条件
试剂盒于-30 ~ -15℃保存,≤0℃运输;避免强光直射。
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