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- 详细信息
- 文献和实验
- 技术资料
- 库存:
充足
- 英文名:
Add&Read® Mouse Fc Kit
- 供应商:
南京诺唯赞生物科技股份有限公司
- 保存条件:
见详情页
- 规格:
500 tests/10,000 tests
产品描述
Mouse Fc Kit可用于高通量检测细胞上清中或者纯化后的Mouse IgG或者mFc融合蛋白(mFc -fusion protein)的浓度。试剂盒中有一株抗体识别Mouse IgG的Fc区域,标记荧光供体Eu(Anti-Mouse IgG-Eu);还有一株Mouse IgG,标记荧光受体A2(Mouse IgG-A2)。
在溶液中,Anti-Mouse IgG-Eu与Mouse IgG-A2结合,320 nm的光激发荧光供体Eu,Eu供体发射620 nm的光,此620 nm的光激发荧光受体A2,A2受体发射出665 nm的光,此为荧光共振能量转移(FRET)现象。当在溶液中添加待测Mouse IgG或者mFc-fusion protein时,会竞争结合Anti-Mouse IgG-Eu,破坏FRET现象。待测Mouse IgG或者mFc-fusion protein的浓度与FRET的信号值(665 nm/620 nm的光强度比值)成反比。

产品优势
均相检测:免洗ELISA,无需包被、洗板,直接加样-孵育-上机检测,节省人力、时间
节约样本:总体系仅20μl
体系稳定:24h内重复检测,信号值基本不变
高通量筛选:可搭载384孔板进行高通量筛选
产品组分

储存条件
Mouse Fc Kit于-30 ~ -15℃保存,≤0℃运输;
IgG standard冻干粉于2 ~ 8℃保存,加入ddH2O溶解后分装于-30 ~ -15℃保存,避免反复冻融;
Anti-Mouse IgG-Eu和Mouse IgG-A2于-30 ~ -15℃保存,避免反复冻融;
Diluent和Detection buffer于-20 ~ 4℃保存。
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文献和实验together to calculate compensation in multicolor immunophenotyping experiments that incorporate a LIVE/DEAD® fixable dye by following the protocol outlined below: 1) Gently vortex the ArC™ Amine Reactive Beads Kit and the AbC™ Anti-Mouse Bead Kit
A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96
) and centrifuge at 8000-10,000 x g for 1 min at room temperature. Discard the liquid and re-use the collection tube. For volumes greater than 700 ul load the column and centrifuge successively, 700 ul at a time. Each HiBind® extraction column has a total capacity











