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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
无锡傲锐东源生物科技有限公司
- 规格:
0.5 mL
| Product Data | |
| Description | MegaTran 2.0, plasmid DNA transfection reagent (0.5 mL), 330 rxns for 24-well plate |
| Features | » Superior transfection efficiency » Broad cell spectrum » Competitive pricing |
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文献和实验本制品是一种快速方便的总RNA提取试剂,可以从动物组织、植物材料、各种微生物、培养细胞等中提取总RNA。样品在RNAiso Reagent中能够充分被裂解,在加入氯仿离心后,溶液会形成上清层、中间层和有机层(下层),RNA分布在上清层中,收集上清层后,经异丙醇沉淀便可以回收得到总RNA。RNAiso Reagent具有很强的广谱性,可以适用于各种样品的总RNA提取,提取过程方便快速,整个操作在一小时内便可以完成。经本制品提取的总RNA纯度高,基本不含蛋白质及基因组 DNA,提取的RNA可以直接
Procedure for Transfection of Mammalian Cells
in a CO2 incubator until the cells are 70-80% confluent. This will usually take 18-24 h. Prepare the following solutions in 12 x 75 mm sterile tubes: Solution A: For each transfection, dilute 2 μg DNA (plasmid) in 375 μl serum-free IMDM
Preparing Vector DNA For Transfection/Targeting
and transfection, so it is recommended that you at least peruse the sections below to obtain an idea of the level of purity required. Plasmid Prep -Most plasmids are kept in bacteria as glycerol stocks, restreak some out to achieve single colonies. If using
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