- ￥5480
- Sino Biological
- 北京
- 10440-H17H-B-UE
- 2025年11月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human Alkaline Phosphatase / ALPL Protein (AVI Tag), Biotinylated
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
20 µg
蛋白名称：Human Alkaline Phosphatase / ALPL Protein (AVI Tag), Biotinylated, Endotoxin-Free
蛋白构建：A DNA sequence encoding the Human ALPL (NP_000469.3) (Met1-Ser502) was fused with an AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白内毒素：< 0.5 EU per mg protein.
预测N端：Leu 18
蛋白分子量：The recombinant Human ALPL consisting of 500 amino acids and has a calculated molecular mass of 55.21 kDa. As a result of glycosylation, it migrates as an approximately 65-77 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：NP_000469.3
蛋白氨基酸序列：Met1-Ser502
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文献和实验mutations at phenylalanine in a putative PP-1C (the catalytic subunit) binding site motif(K/R)(X)(I/V)XF was unable to rescue Hxk2p dephosphorylation in intact yeast or restore Hxk2p phosphatase activity. These results demonstrate that Reg1p targets PP-1C
Specificity Profiling of Protein‐Binding Domains Using One‐Bead‐One‐Compound Peptide Libraries
) 1 mg/ml SAAP (streptavidin–alkaline phosphatase; Prozyme, http://www.prozyme.com/) Staining buffer (see recipe )
免疫组化技术-Immunohistochemical Protocols
bovine serum albumine (BSA) instead of NGS. The solution should also contain 0.6% Triton X-100 to improve penetration into the tissue. One needs to decide in advance if the end result (final marker or tag) should be fluorescent or suitable
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