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- 规格:
100 µg
蛋白名称:SPF30蛋白, SPF30 protein
蛋白构建:A DNA sequence encoding the mature form of human SMNDC1 (O75940) (Met1-Gln238) was expressed with a polyhistide tag at the N-terminus.
表达宿主:E. coli
蛋白纯度:> 85 % as determined by SDS-PAGE
蛋白活性:0
蛋白内毒素:Please contact us for more information.
预测N端:His
蛋白分子量:The recombinant human SMNDC1 consists of 253 amino acids and predicts a molecular mass of 28.5 KDa. It migrates as an approximately 29 KDa band in SDS-PAGE under reducing conditions.
蛋白NP号:O75940
蛋白氨基酸序列:Met1-Gln238
蛋白标签:N-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验为His-tag 与 Strep-tag® 系统的耐受缓冲液对比 如果分离纯化膜蛋白或包涵体时,则纯化过程需要涉及到变性条件。 Strep-tag®系统最多允许的尿素浓度为6M,盐酸胍为1M,而His-tag 则为8M和6M。如若需要增加蛋白的溶解度,使用去垢剂,则两种系统均可兼容Triton-X100,Tween 20和Nonidet P40。 但不是所有的蛋白过表达后都在包涵体内,且膜蛋白仅占细胞表达蛋白的20%-30%。 因此,如果纯化过程需要考虑特殊的纯化条件,两种系统是都可以选择的。但如果比较
DNA Directed in Vitro Protein Synthesis with Escherichia coli S-30 Extracts
A DNA-directed cell-free protein synthesizing system was originally developed by Zubay (1 ). The system contains a crude extract prepared from Escherichia coli . This extract contains the machinery necessary for the transcription
偶然发现的一个pQE30系统表达蛋白纯化案例,帖出来和大家分享。 Purification of LF. 诱导与表达 E. coli SG13009(pREP4) carrying the recombinant plasmid pPG-LF1 was grown at 37°C in Luria broth with 100 μg of ampicillin and 25 μg of kanamycin per ml at 250 rpm. When the A600
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