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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Human HER2 / ErbB2 Protein (His Tag), Endotoxin-Free
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
100.00 µg/1.00 mg
| 规格: | 100.00 µg | 产品价格: | ¥3490.0 |
|---|---|---|---|
| 规格: | 1.00 mg | 产品价格: | ¥22670.0 |
蛋白名称:重组人HER2 / ErbB2蛋白(His标签)
蛋白构建:A DNA sequence encoding the extracellular domain (Met 1-Thr 652) of human ErbB2 (NP_004439.2) was fused with the polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS(Routinely tested).
蛋白活性:1.Immobilized Recombinant Human HER2 / ErbB2 Protein (His Tag)(Cat: 10004-H08H-UE ) at 2 μg/mL (100 μL/well) can bind Anti-HER2 (Research Grade Trastuzu * mab Biosimilar), the EC50 is 5-15 ng/mL (Routinely tested). 2.Loaded Anti-HER2 (Research Grade Pertuzumab Biosimilar) on proA Biosensor, can bind Recombinant Human HER2 / ErbB2 Protein (His Tag) (Cat. No. 10004-H08H-UE) with an affinity constant of 2.29 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 3.Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation(Routinely tested).
蛋白内毒素:< 0.5 EU per mg protein.
预测N端:Thr 23
蛋白分子量:The recombinant human ErbB2 comprises 641 amino acids and has a calculated molecular mass of 71 kDa. As a result of glycosylation, the band migrates approximately 80-100 kDa in the SDS-PAGE under reducing conditions. The molecular weight of this protein is around 86.7 kDa verified by SEC-MALS(Routinely tested).
蛋白NP号:NP_004439.2
蛋白氨基酸序列:Met 1-Thr 652
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文献和实验His 标签蛋白纯化效果不理想,宝宝心里苦呀。今天,小编来跟大家一起找找原因。 纯化所得组分中没有收集到重组的His标签蛋白。该问题主要可以分为以下两个方面: 1、His标签蛋白没有结合到填料上就流穿了 原因一 超声的功率不对。超声功率过高,容易导致蛋白炭化;功率过低,蛋白释放不出来。 建议 改变超声功率,同时可以在超声前加入溶菌酶。 原因二 结合缓冲液条件不合适。 建议 检查结合缓冲液中是否有影响结合的因素,如:金属离子螯合剂、强还原剂、过高的咪唑浓度。同时,优化缓冲液的pH、盐
植物源重组人胰岛素生长因子融合蛋白(OsrIGF-1),胰岛素在无血清细胞培养中的最佳替代品。 IGFs家族由两种低分子多肽(IGF-Ⅰ、IGF-Ⅱ)、两类特异性受体及六种结合蛋白组成。IGF-Ⅰ是一个有70个氨基酸的单链碱性蛋白,分子量7649Da,耐热。IGFs的生物学功能不只局限于有丝分裂刺激作用,它们也能诱导分化或促进分化功能的表达。全新推出的植物源重组人类胰岛素生长因子-1融合蛋白(OsrIGF-1)在IGF-1的N末端融合了人血清白蛋白(HSA),HSA作为蛋白质保护剂增加了IGF
Assay for Isolation of Inhibitors of Her2-Kinase Expression
Her2 (ErbB2) protein is overexpressed in breast and other solid tumors, and its expression is associated with progressive disease. Current therapies directed toward Her2 either block dimerization of the receptor or inhibit tyrosine kinase
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