重组小鼠FCGRT & B2M Heterodimer蛋白

Recombinant Mouse FCGRT & B2M Heterodimer Protein（产品说明）
蛋白名称：重组小鼠FCGRT & B2M Heterodimer蛋白
蛋白构建：A DNA sequence encoding the extracellular domain (Met 1-Ser 297) of mouse FCGRT (Q61559) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the mouse B2M (P01887,105Ala/Asp) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT / B2M heterodimer was purified
表达宿主：HEK293 Cells
蛋白纯度：≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. ≥ 95 % as determined by SEC-MALS(Routinely tested).
蛋白活性：1.Loaded Mouse FcRn recombinant protein (Cat. CT029-M08H-UE) on His1k Biosensor, can bind Anti-VEGF (Research Grade Bevacizumab Biosimilar) with an affinity constant of 1.14 nM as determined in a BLI assay (Sartorius Octet Red384) (Routinely tested). 2.Recombinant Mouse FCGRT & B2M Heterodimer Protein (Cat: CT029-M08H-UE) captured on CM5 chip via Anti-His antibody, can bind Anti-VEGF (Research Grade Bevacizumab Biosimilar) with an affinity constant of 20.790 nM as determined in an SPR assay (Biacore T200) (Routinely tested).
蛋白内毒素：< 0.5 EU per mg protein.
预测N端：Ala 24 & Ile 21
蛋白分子量：The recombinant heterodimer of mouse FCGRT / B2M comprises 386 (287+99) amino acids and has a calculated molecular mass of 44.17 ( 32.48 + 11.69 ) kDa. The bands migrate to 10-15 kDa and 37-50 kDa in the SDS-PAGE under reducing conditions. The molecular weight of this protein is around 53.9 kDa verified by SEC-MALS(Routinely tested).
蛋白NP号：Q61559 & P01887
蛋白氨基酸序列：Met1-Ser297 & Met1-Met119