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- 规格:
50 µg
蛋白名称:SR-BI/SCARB1蛋白, SR-BI/SCARB1 protein
蛋白构建:A DNA sequence encoding the mature form of mouse SRB1 (NP_058021.1) extracellular domain (Pro 33-Val 440) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Pro 33
蛋白分子量:The secreted recombinant mouse SRB1/Fc is a disulfide-linked homodimer The reduced monomer comprises 656 amino acids with a predicted molecular mass of 74 kDa. As a result of glycosylation, it migrates as an approximately 115-120 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:NP_058021.1
蛋白氨基酸序列:Pro33-Val440
蛋白标签:C-human IgG1-Fc & His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
实验原理 If using the SQ DNA/RNA/Protein for the first time, please read this booklet to become familiar with the procedure and its various modifications. Samples are first lysed in a specially formulated buffer. The protein
,于室温处理 1 小时进行终止。+4°C 环境下,将神经元细胞与神经元标志微管相关蛋白 2 (microtubule-associated protein 2,MAP-2) (英国剑桥Abcam,ab11267, HM-2,1:600 稀释)小鼠单克隆抗体共同孵育,孵育过夜。第 2 天,将细胞与山羊抗小鼠二抗(Alexa Fluor® 488 goat anti-mouse IgG (H+L) (Invitrogen,1:250 稀释)共同孵育。使用DMI6000 B 倒置显微镜(德国,Leica
圆括号中所标的物种的 IgG 和 / 或血清蛋白的吸附处理的)。当其他物种免疫球蛋白的出现可能导致交叉反应时,推荐使用这类抗体。但是,当要选择经过针对相近物种吸附处理的抗体时,要特别注意,因为它们能识别的表位数量大大减小了,可能识别单抗的能力很弱。例如,在含有内源性免疫球蛋白的大鼠的组织样本中,只用经大鼠 IgG 吸附处理的抗鼠 IgG 检测大鼠组织中的鼠源一抗,或者在多标记实验中使用了大鼠的一抗。另个一个例子是抗大鼠 IgG(min X Mouse… Sr Prot) 和抗叙利亚仓鼠 IgG
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