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文献和实验v468 Chapter 9 自身启动子报告基因验证转录靶点
Native Promoter Reporters Validate Transcriptional Targets Methods in Molecular Biology v468. chapter 9 Otto Schmalhofer, Simone Spaderna ,and Thomas Brabletz Abstract The transcriptional activator b-catenin is the key mediator
(29:1 ratio; 40% stock solution) (Sigma) (for hot-stop PCR only; see Steps 1-22)Agarose (for duplex PCR only; see Steps 40-53)Ammonium persulfate (APS; 10%, w/v), freshly prepared (for hot-stop PCR [Steps 1-22] or SSCP [Steps 23-37] only)α-32PdCTP (10
specificity has been termed “star” activity. It has been suggested that star activity may be a general property of restriction endonucleases (1) and that any restriction endonuclease can be made to cleave noncanonical sites under certain extreme conditions
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