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依诺赞(江苏)生物科技有限公司
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50rxns
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文献和实验Isolation of cell nuclei for the application in the cell-free system
from the surrounding cytoskeleton during cell lysis. For the preparation of Jurkat nuclei, the step of cytochalasin treatment can be omitted. Input of about 1E+07 cells. Lysis in hypotonic medium (about 23 mM compared with 137 mM in isotonic medium) using a Dounce
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Preparation of Cell-Free Extracts from Mycobacteria
modification, such as glycosylation (15 ,16 ). Because glycosylation does not occur in E. coli , the study of such proteins in their native state requires the use of mycobacterial hosts. The preparation of cell-free extracts and protein purification
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