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- EN0141
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
1年
- 英文名：
Endonuclease V, T.maritima
- 供应商：
北京智杰方远科技
- 保存条件：
详见说明
- 规格：
250 units
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文献和实验The Use of Resolvases T4 Endonuclease VII and T7 Endonuclease I in Mutation Detection
, then heteroduplex species will be generated with a base-pair mismatch at that position (see Fig. 1 ). These mismatches can be bound and the DNA cleaved by at least two resolvase enzymes, T4 endonuclease VII (1 ) and T7 endonuclease I (3 ), both bacteriophage
Partial proteolysis with Staph. aureus V8 protease
. If you have too much, the V8 won't work. The molarity of neat mercaptoethanol is approximately 14. 1 microliter of ME per 14 ml of solution gives a 1 mmolar solution. Enzyme stock solution: 500 micrograms/ml (or 500 nanograms/microliter) V8-protease in 125
Partial Endonuclease Digestion
, tubes 2 through 4 contain 20 µl, and tube 5 contains 10 µl (see diagram below). Place the tubes on ice. Add restriction endonuclease (3-10 units of enzyme per µg of DNA) to tube 1, mix quickly, and place the tube on ice. Add 10 µl
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