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- SPEX CertiPrep
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- ZSC-ECS-N-005
- 2025年07月14日
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- 文献和实验
- 技术资料
- 保存条件：
保存：Refrigerator
- 保质期：
咨询甄准
- 英文名：
Base/Neutral Spiking Mix (1000ppm) - 5.5mL Bottle
- 库存：
咨询甄准
- 供应商：
上海甄准
- 规格：
5.5 mL
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文献和实验off with 0.05% Tween 20 in PBS. 7. Block for about 1 hr with 5% BSA/Tween 20, or overnight with 3% BSA/Tween 20. Reagents and Buffers for Westerns Reagent 30% acrylamide/bisacrylamide (29:1 mix) 100ml(dark bottle at 4°C) 29g acrlamid 1g bisacrylamid
the chromosomal DNA). 4. Add a total of 140 ml of alkaline lysis solution (70 ml for each bottle), gently mix, and incubate for 5 minutes in an ice-water bath. 5. Add 105 ml of 3M NaOAc, pH 4.8 (52.5 ml for each bottle), cap tightly, gently mix
to mix together and then add to agar to make plates. Making amino acid dropout (CSM) solution Generally easiest to make a 10x master solution that you can dilute back when you''re pouring plates. Let''s assume you''re going to make 200ml
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