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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20C
- 英文名:
TAXANE (TAXOL) MIXTURE(2 Compound Mix)[0.05mg/mL](P) (In Solution)
- 供应商:
上海汇谱仪器有限公司
- 规格:
1mL
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文献和实验Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC
fluorescent polarity marked taxol-stabilized microtubules to determine polarity, as described later. 1. Coat flow cell with a mixture of 0.25 mg/ml casein and kinesin (5-50 µg/ml) for 3'.. 2. Rinse out unbound material with 80 µl BRB80 + 1 mM DTT
Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC
with a mixture of 0.25 mg/ml casein and kinesin (5-50 µg/ml) for 3'. 2. Rinse out unbound material with 80 µl BRB80 + 1 mM DTT + 1 mM MgATP + 10 µM taxol + 1X OS Mix. 3. Flow in 30-50 µl of 10 ug/ml polarity marked taxol microtubules diluted from a 1
In Situ HYBRIDIZATION TO TISSUE SECTIONS
Hybridization Pretreat the slides. Mix probe with appropriate buffer. Place hyb mixture onto the slides: Lay slides horizontally on a black surface. Apply 10-15ml probe solution along- side one edge of the tissue. Using forceps, carefully
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