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- SPEX CertiPrep
- 国外
- ZSC-ECS-N-027
- 2025年07月15日
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- 文献和实验
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- 保存条件：
保存：Refrigerator
- 保质期：
咨询甄准
- 英文名：
Semivolatile Surrogate Mix (6) (1000/2000ppm) - 5.5mL Bottle
- 库存：
咨询甄准
- 供应商：
上海甄准
- 规格：
5.5 mL
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文献和实验the chromosomal DNA). 4. Add a total of 140 ml of alkaline lysis solution (70 ml for each bottle), gently mix, and incubate for 5 minutes in an ice-water bath. 5. Add 105 ml of 3M NaOAc, pH 4.8 (52.5 ml for each bottle), cap tightly, gently mix
IMMUNOHISTOCHEMISTRY ON WHOLE MOUNT EMBR
, although my current view is that this is not necessary. I use Tago G anti- Ms, Rb and Rt. I use HRP-avidin and AP-avidin from BRL @ 1:2000 and 1:1000, respectively. Add avidin-enzyme conjugate in PBT, rotate 30''. Wash 3X in PBT with a final wash in AP
IMMUNOHISTOCHEMISTRY ON WHOLE MOUNT EMBRYOS
on a Millipore device with a nitex filter; rinse well with 0.1% Triton X-100 from a squirt bottle. Transfer embryos to 3:1 heptane:fix with a paint brush and fix for 20-40 min. Fix in a glass scintillation vial using 6.1ml HEPES Fix Mix + 0.75ml 37
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