Miltenyi货号130-045-201人CD8磁珠(hu

Miltenyi货号130-045-201人CD8磁珠(human CD8 MicroBeads)上海睿安生物13611631389

该试剂盒用于从不同组织(如外周血和脐血)中分离细胞毒性CD8T细胞。另外，细胞毒性CD8T细胞还可以从淋巴结、胸腺、脾脏等组织中分离.

CD8抗原与T细胞受体形成复合物，并在TCR异二聚体对CD8+细胞毒性T细胞的MHC I类/肽复合物的识别中充当辅助分子。 CD8分子由α/β异二聚体或α/α同二聚体组成。它在细胞毒性T细胞上强表达，而在NK细胞的一部分上却弱表达。CD8存在于大多数胸腺细胞和占所有外周血T细胞约三分之一的。 CD8 +细胞毒性T细胞在杀死病毒感染的细胞和肿瘤细胞中起重要作用。

下游应用:

CD8+细胞毒性T细胞的分离或去除在许多不同的研究领域中进行，例如传染病，自身免疫性疾病，变态反应和哮喘以及肿瘤免疫学。通过MACS 技术分离出的细胞毒性T细胞仍然具有活力和功能。因此，它们可用于进一步的功能研究，例如增殖测定和细胞毒性测定，还可用于体外细胞因子产生的分析。 CD8磁珠也已用于去除人PBMC的CD8 + T细胞，用于SCID小鼠的免疫重建实验。与CD4多次分选磁珠结合使用，CD8磁珠已用于分离CD4 + CD8 +双阳性胸腺细胞。其他例子包括有关病毒感染的各种研究，例如CD8⁺细胞在体外感染HIV6或监测受HIV感染的母亲的孩子的免疫异常。

优点:

-简单、快速；

-高效。

组成成分: ▪人CD8磁珠:与人CD8单克隆抗体(同型:小鼠IgG2a)偶联的磁珠，2ml

相关产品:

▪MS分选柱(Miltenyi货号130-042-201)

▪LS分选柱(Miltenyi货号130-042-401)

▪磁力架(Miltenyi货号130-042-303)

▪MS分选磁极(Miltenyi货号130-042-102)

▪Rinsing solution(Miltenyi货号130-091-222)

▪Running buffer(Miltenyi货号130-091-221)

Specifications for CD8 MicroBeads, human
Overview
CD8 MicroBeads were developed for the positive selection or depletion of human cytotoxic CD8+ T cells from different cell sources. The most commonly used cell sources are peripheral blood and cord blood. In addition, cytotoxic T cells have also been isolated from lymph nodes, thymus, skin biopsies1, and spleen.

Detailed product information
Background information
The CD8 antigen forms a complex together with the T cell receptor and acts as an accessory molecule in the recognition of MHC class I/peptide complexes by the TCR heterodimer on CD8+ cytotoxic T cells. The CD8 molecule consists of either an α/β heterodimer or an α/α homodimer. It is expressed strongly on cytotoxic T cells and dimly on a subset of NK cells. CD8 is found on most thymocytes and on about one third of all peripheral blood T cells. CD8+ cytotoxic T cells play an important role in the killing of virus-infected cells and tumor cells.

Downstream applications
Isolation or depletion of CD8+ cytotoxic T cells is performed in many different research fields such as infectious diseases, autoimmune diseases, allergy, and asthma, as well as tumor immunology.

Cytotoxic T cells isolated by MACS® Technology remain viable and functional. Therefore, they can be used for further functional studies, such as proliferation assays2,9 and cytotoxicity assays2,3, but also for the analysis of in vitro cytokine production4. CD8 MicroBeads have also been used for the depletion of CD8+ T cells from human PBMCs for immune reconstitution experiments in SCID mice.5

In combination with CD4 MultiSort MicroBeads, CD8 MicroBeads have been used for the isolation of CD4+CD8+ double-positive thymocytes.8

Other examples include various studies on viral infections, e.g., in vitro infections of CD8+ cells with HIV6, or monitoring of immune abnormalities in children of HIV-infected mothers7.

Columns
For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, D, or autoMACS Columns.

References for CD8 MicroBeads,human
Publications
Akdis, M. et al. (1999) Skin homing (cutaneous lymphocyte-associated antigen-positive) CD8+ T cells respond to superantigen and contribute to eosinophilia and IgE production in atopic dermatitis. J Immunol 163: 466-475
Walker, W. and Gallagher, G. (1994) The in vivo production of specific human antibodies by vaccination of human-PBL-SCID mice. Immunology 83: 163-170
Turner, J. and Dockrell, H. M. (1996) Stimulation of human peripheral blood mononuclear cells with live Mycobacterium_bovis BCG activates cytolytic CD8+ T cells in vitro. Immunology 87: 339-342
Kutlesa, S. et al. (2002) Developmentally regulated interactions of human thymocytes with different laminin isoforms. Immunology 105: 407-418
Al-Shanti, N. et al. (2004) Investigation of alpha nascent polypeptide-associated complex functions in a human CD8+ T cell ex vivo expansion model using antisense oligonucleotides. Immunology 112: 397-403
Akdis, A. C. et al. (1995) Cytokine immunotrapping: an assay to study the kinetics of production and consumption or degradation of human interferon-gamma. J. Immunol. Methods 182: 251-261
Parra, E. et al. (1997) The role of B7-1 and LFA-3 in costimulation of CD8+ T cells. J Immunol 158: 637-642
Nielsen, S. D. et al. (2001) Impaired progenitor cell function in HIV-negative infants of HIV-positive mothers results in decreased thymic output and low CD4 counts. Blood 98: 398-404
Ennen, J. et al. (1994) CD8+ T lymphocytes of African green monkeys secrete an immunodeficiency virus-suppressing lymphokine. Proc. Natl. Acad. Sci. U.S.A. 91: 7207-7211