美天旎Miltenyi货号130-095-838小鼠记忆B细

美天旎Miltenyi货号130-095-838小鼠记忆B细胞分离试剂盒(Memory B Cell Isolation Kit，mouse)上海睿安生物13611631389

记忆B细胞富集试剂盒可从小鼠脾脏和淋巴结单细胞悬液中分离记忆B细胞。

记忆B细胞是源自抗原经历的前体增殖的静止B细胞。 它们能够快速对抗原再次攻击作出反应，从而提供体液免疫保护。

详细的分离过程

小鼠记忆B细胞的分离分两个步骤进行:首先，非记忆B细胞被生物素偶联抗体混合物标记，然后使用抗-生物素磁珠标记这些细胞进行去除。与混合物标记的同时，细胞还分别用IgG1-APC和IgG2ab-APC抗体标记。第二步，将预富集的记忆B细胞用抗-APC磁珠标记，并在MACS分选柱上进行阳性分选。

Specifications for Memory B Cell Isolation Kit, mouse
Overview
The Memory B Cell Isolation Kit was developed for the isolation of memory B cells from mouse spleen and lymph nodes.

Detailed product information
Background information
Memory B cells are quiescent B cells derived from proliferating antigen-experienced precursors. They are able to quickly react to a recurrent antigenic challenge, thereby providing humoral immune protection.

Detailed separation procedure
The isolation of mouse memory B cells is performed in two steps: First, non-memory B cells are indirectly magnetically labeled with a cocktail of biotin-conjugated antibodies, as primary labeling reagent and subsequently depleted using Anti-Biotin MicroBeads. During the first incubation step with the cocktail, cells are additionally labeled with Anti-IgG1-APC and Anti-IgG2ab-APC antibodies. In the second step, pre-enriched memory B cells are labeled with Anti-APC MicroBeads and isolated by positive selection over a MACS Column.

Columns:

For the first magnetic separation (depletion):LD Columns. For the second magnetic separation (positive selection): MS Columns.

Product data and images for Memory B Cell Isolation Kit, mouse
Figure 1
Memory B cells were isolated from mouse spleen using the Memory B Cell Isolation Kit, mouse. Cells were fluorescently stained with CD45R (B220)-VioBlue (#130-094-287), CD38-PE (#130-097-087) and Anti-IgM-FITC (#130-095-906) and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

图1 使用小鼠记忆B细胞分离试剂盒，从小鼠脾中分离记忆B细胞。用CD45R(B220)-VioBlue(美天旎Miltenyi货号130-094-287)，CD38-PE(美天旎Miltenyi货号130-097-087)和IgM-FITC(美天旎Miltenyi货号130-095-906)染色细胞并使用MACSQuant分析仪进行流式分析。基于散射信号(SSC)和碘化丙啶(PI)排除细胞碎片和死细胞。

▪MS分选柱(美天旎Miltenyi货号130-042-201)

▪LS分选柱(美天旎Miltenyi货号130-042-401)

▪磁力架(美天旎Miltenyi货号130-042-303)

▪MS分选磁极(美天旎Miltenyi货号130-042-102)

▪Rinsing solution(美天旎Miltenyi货号130-091-222)

▪Running buffer(美天旎Miltenyi货号130-091-221)