- ￥1838
- BioLegend
- 400102
- 美国
- 2025年07月15日
- 免疫荧光，流式
- 小鼠
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Purified Mouse IgG1, κ Isotype Ctrl
- 抗体英文名：
Purified Mouse IgG1, κ Isotype Ctrl
- 浓度：
0.5 mg/mL
- 应用范围：
免疫荧光，流式
- 宿主：
小鼠
- 供应商：
上海善然生物
- 库存：
现货
- 抗原来源：
小鼠
- 保质期：
2-3年左右
- 标记物：
Purified Mouse IgG1, κ Isotype Ctrl
- 克隆性：
单克隆
- 保存条件：
2-8度
- 规格：
500 μg
Purified Mouse IgG1, κ Isotype Ctrl Antibody
MOPC-21
Catalog# / Size 400101 / 50 µg
400102 / 500 µg
Clone
Regulatory Status RUO
Isotype Mouse IgG1, κ
Description The MOPC-21 immunoglobulin has unknown specificity. The isotype of this antibody is mouse
IgG1, κ. This antibody was chosen as an isotype control after screening on a variety of resting,
activated, live, and fixed mouse, rat and human tissues.
Product Details
Antibody Type Monoclonal
Host Species Mouse
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation The immunoglobulin was purified by affinity chromatography.
Concentration 0.5 mg/ml
Storage & Handling The immunoglobulin solution should be stored undiluted between 2°C and 8°C
Application FC, ICFC - Quality tested
ChIP - Verified
ICC, IHC, IP, WB, FA - Reported in the literature, not verified in house
Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent staining with flow
cytometric analysis as negative control. Use at concentrations comparable to those of the specific
antibody of interest. Use our Concentration Lookup tool to find the exact concentrations of your
lots of product.
Application Notes Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry
(ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP),
Western Blotting (WB), Functional Assay (FA)
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Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
on mouse tissue are of IgG1 isotype. Detailed explanation of this method can be found in: Cattoretti G, Qing Fei. Application of the Antigen Retrieval Technique in experimental Pathology: from human to mouse
Rapid Isotyping of Mouse Monoclonal Antibodies
Determination of class and subclass of monoclonal antibodies (MAbs) is important because several immunochemical and functional properties of antibodies depend on the isotype. Knowledge of the light-chain isotype (κ for 95% or λ
Immunofluorescent Staining of Mouse and Rat Leukocytes
. Examples of negative controls are unstained cells, cells exposed to the 2nd step reagent alone, or cells exposed to isotype controls which are the same isotype and format (e.g.,purified, biotin or fluorochrome) as the primary antibody and titrated
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