2-Butyl-1,2-Benzisothiazolin-3

疏水相互作用层析

高盐蛋白的分离   基本步骤 疏水作用层析作为一种吸附性层析，主要步骤包括平衡、上样、清洗、洗脱和再生步骤。疏水作用层析一般在高盐环境中上样，经过上样和清洗之后，通过降低洗脱液中的盐浓度逐渐对不同的分子进行洗脱。其流程可参考下图（图13）： 图13 疏水作用层析流程图   填料选择 疏水填料和离子交换填料类似，都是由基架和疏水配基交联而成，常见的疏水填料配基有 Phenyl、Butyl、Octyl 等，常见疏水填料强弱如图所示： 图14 常见疏水填料强弱图   疏水性强弱，在实验初期

Solid-Phase Synthesis of Neuropeptides by Fmoc Strategies

is used to protect the α-amino group of each residue. Those residues that have potentially reactive side chains are protected with acid-labile groups such as t-butyl. After removal of the Fmoc group with piperidine, the next protected amino

Synthesis of 2-O-Alkyloligoribonucleotides

7 ), ethyl (8 ), allyl (9 ), or butyl (10 ), with most studies being carried out with the methyl and allyl analogs.