DNA-Free Thermostable Alkaline

DNA-Free Thermostable Alkaline Phosphatase catalyzes the hydrolysis of phosphate monoester bonds but does not catalyze the hydrolysis of phosphate diester or phosphate triester bonds. This enzyme is a high-temperature enzyme, exhibiting high activity at 60-100℃ and cannot be inactivated by heat. The specific activity of this enzyme is approximately 20 U/mg.

Cat. No.: DFMAP-500 (for 500U)

Cat. No.: DFMAP-2500 (for 2500U)

Description

DNA-Free Thermostable Alkaline Phosphatase catalyzes the hydrolysis of phosphate monoester bonds but does not catalyze the hydrolysis of phosphate diester or phosphate triester bonds. This enzyme is a high-temperature enzyme, exhibiting high activity at 60-100℃ and cannot be inactivated by heat. The specific activity of this enzyme is approximately 20 U/mg.

Our DNA-Free enzymes are all recombinant proteins and purified through multiple steps, devoid of genomic and plasmid DNA from the production strain.

Unit Definition

One unit is defined as the amount of enzyme required to hydrolyze 1 μmol of p-nitrophenyl phosphate to p-nitrophenol within 1 minute at 75℃ in 1× Alkaline Phosphatase reaction buffer.

Features

• Completely free from bacterial DNA.
• Removal of 5’ phosphate groups from DNA molecules.
• Pre-treatment of PCR products for sequencing when combined with Exonuclease I.

Storage

Store at -20℃ for up to 2 years. Avoid repeated freeze-thaw cycles.