DNA-Free PAGE Taq DNA Polymera

Taq DNA Polymerase is a thermostable Taq DNA polymerase derived from the thermophilic bacterium Thermus aquaticus, recombinantly expressed in Escherichia coli, with a molecular weight of 94 kDa. This enzyme is specially modified for enhanced amplification capability and extension speed, achieving fragment lengths of up to 5-6 kb with an extension rate of 2-3 kb/min (72°C). It exhibits 5'→3' polymerase activity, weak 5'→3' exonuclease activity, and no 3'→5' exonuclease activity. Amplification products have 3'-dA overhangs. PAGE Taq DNA Polymerase is supplied with a specially formulated buffer, making its PCR products suitable for polyacrylamide gel electrophoresis and agarose gel electrophoresis.

Cat. No.: DFPGT-500 (for 500U)

Cat. No.: DFPGT-2500 (for 2500U)

Cat. No.: DFPGT-10k (for 10KU)

Cat. No.: DFPGT-25k (for 25KU)

Description

Taq DNA Polymerase is a thermostable Taq DNA polymerase derived from the thermophilic bacterium Thermus aquaticus, recombinantly expressed in Escherichia coli, with a molecular weight of 94 kDa. This enzyme is specially modified for enhanced amplification capability and extension speed, achieving fragment lengths of up to 5-6 kb with an extension rate of 2-3 kb/min (72°C). It exhibits 5'→3' polymerase activity, weak 5'→3' exonuclease activity, and no 3'→5' exonuclease activity. Amplification products have 3'-dA overhangs. PAGE Taq DNA Polymerase is supplied with a specially formulated buffer, making its PCR products suitable for polyacrylamide gel electrophoresis and agarose gel electrophoresis.

Our DNA-Free PAGE Taq DNA Polymerase is devoid of genomic and plasmid DNA from the production strain. This makes this polymerase particularly suitable for amplifying, sequencing, and cloning 16S and 23S rDNA genes, as well as for standard applications like PCR and primer extension.

Features

• Completely free from bacterial DNA.

• Specially modified for enhanced amplification capability, effectively amplifying 3-5kb DNA fragments at a rate of 2kb/min.

• Excellent thermal stability: Half-life exceeds 40 minutes at 95°C.

• PCR products have 3'-dA overhangs, making them directly suitable for T/A cloning.

• Broad tolerance range for template DNA.

• Can incorporate dUTP, dITP, and fluorescently labeled nucleotides.

Applications

• Conventional PCR amplification.

• Colony PCR.

• T/A cloning with PCR products having 3'-dA overhangs.

• DNA fluorescence labeling.

Unit Definition

The amount of enzyme required to incorporate 10 nmol of deoxyribonucleotide into acid-insoluble material within 30 minutes at 72°C, using salmon sperm DNA as the template/primer, is defined as one activity unit (U).

Storage