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- DFT7R-5k (for 5KU)
- 2026年01月07日
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DNA-Free T7 RNA Polymerase is a DNA-dependent RNA polymerase encoded by the T7 bacteriophage, used for in vitro RNA synthesis. It has high specificity for the T7 phage promoter. Under the guidance of the T7 promoter, it uses DNA as a template to synthesize either sense or antisense RNA, depending on the position of the T7 promoter relative to the DNA sequence. If the DNA is downstream of the T7 promoter, T7 RNA polymerase will transcribe sense RNA; otherwise, it will transcribe antisense RNA. RNA synthesized in vitro using T7 RNA polymerase is suitable for various research and biotechnology applications, such as sgRNA for gene editing and mRNA synthesis for in vitro translation. Both double-stranded linear blunt-ended or 5’ overhang-ended DNA can serve as substrate templates for T7 RNA polymerase, making linear plasmids and PCR products suitable templates for in vitro RNA synthesis.
Cat. No.: DFT7R-5k (for 5KU)
Cat. No.: DFT7R-25k (for 25KU)
Cat. No.: DFT7R-100k (for 100KU)
Cat. No.: DFT7R-250k (for 250KU)
Description
DNA-Free T7 RNA Polymerase is a DNA-dependent RNA polymerase encoded by the T7 bacteriophage, used for in vitro RNA synthesis. It has high specificity for the T7 phage promoter. Under the guidance of the T7 promoter, it uses DNA as a template to synthesize either sense or antisense RNA, depending on the position of the T7 promoter relative to the DNA sequence. If the DNA is downstream of the T7 promoter, T7 RNA polymerase will transcribe sense RNA; otherwise, it will transcribe antisense RNA. RNA synthesized in vitro using T7 RNA polymerase is suitable for various research and biotechnology applications, such as sgRNA for gene editing and mRNA synthesis for in vitro translation. Both double-stranded linear blunt-ended or 5’ overhang-ended DNA can serve as substrate templates for T7 RNA polymerase, making linear plasmids and PCR products suitable templates for in vitro RNA synthesis.
Our DNA-Free T7 RNA Polymerase is a recombinant protein and purified through multiple steps, devoid of genomic and plasmid DNA from the production strain.
Unit Definition
One unit is defined as the amount of enzyme required to incorporate 1 nmol ATP into acid-insoluble material within 60 minutes at 37℃ in 1× T7 RNA polymerase reaction buffer.
Features
- Completely free from bacterial DNA.
- High transcriptional activity, NTP concentration range: 0.2-10 mM.
- The T7 promoter is short and simple, making it easy to design and synthesize.
Applications
- In vitro transcription for the preparation of various RNA molecules, including mRNA, sgRNA, RNA standards, and RNA vaccine synthesis.
- Preparation of radiolabeled and fluorescently labeled RNA probes.
- Expression regulation through antisense RNA.
- Guide RNA (sgRNA) for gene editing.
- Synthesis of mRNA for in vitro translation and microinjection.
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文献和实验mRNA Amplification with T7 RNA Polymerase
on a 42ºC oven and set PCR machine to hold at 70ºC. In an Eppendorf tube add: 1 µg Total RNA 1 µL T7 oligo(dT) primer q.s. to 12 µL with Nuclease-free H2 O Incubate 10 min. at 70ºC. Spin briefly to pull
Oocyte Expression With Injection of Purified T7 RNA Polymerase
of complementary DNA (cDNA). We report on a third expression technique that is based on the combined injection of cDNA and purified T7 RNA polymerase directly into the cytoplasm of oocytes.
In Situ Labeling of DNA Breaks and Apoptosis by T7 DNA Polymerase
The native T7 DNA polymerase is a fast and highly processive enzyme that can be used for in situ detection of apoptosis and various types of DNA breaks. The technique is quick and simple, and was shown to label earlier stages
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