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- DFT4G32-200 (for 200 μg)
- 2026年01月05日
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T4 Gene 32 Protein is a single-stranded DNA binding protein required for bacteriophage T4 replication and repair. It coordinately binds and stabilizes the transiently formed ssDNA region and plays an important structural role in the process of T4 phage DNA replication. The protein has also been widely used to stabilize and label ssDNA regions in order to observe the structure of intracellular DNA with electron microscopy. This product does not contain glycerol which can be used to establish a freeze-drying system.
Cat. No.: DFT4G32-200 (for 200 μg)
Cat. No.: DFT4G32-1k (for 1 mg)
Cat. No.: DFT4G32-4k (for 4 mg)
Description
T4 Gene 32 Protein is a single-stranded DNA binding protein required for bacteriophage T4 replication and repair. It coordinately binds and stabilizes the transiently formed ssDNA region and plays an important structural role in the process of T4 phage DNA replication. The protein has also been widely used to stabilize and label ssDNA regions in order to observe the structure of intracellular DNA with electron microscopy.
Our DNA-Free T4 Gene 32 Protein, on the other hand, is devoid of genomic and plasmid DNA from the production strain. This makes DNA-free T4 Gene 32 Protein particularly suitable for amplifying, sequencing, and cloning 16S and 23S rDNA genes, as well as for standard applications like PCR and primer extension.
Applications
- Increase yield and processivity of reverse transcriptase during RT-PCR
- Stabilize and label ssDNA structure
- Increase yield and specificity of PCR products from soil samples
Thermal Inactivation
65°C, 20 min.
Storage
The minimum shelf life is 3 years at -20°C
Notes
- The molecular weight of this protein is 33 KDa.
- This product does not contain glycerol and thus can be used for the establishment of the lyophilized system.
- The optimal reaction temperature of the protein is 37°C.
Reagents for Recombinase Polymerase Amplification (RPA)
- T4 UvsX Recombinase
- T4 UvsY Protein
- T4 Gene 32 Protein
- Bsu DNA Polymerase
- Sau DNA Polymerase
- Exonuclease III
- Bst DNA/RNA Polymerase
- Endonuclease IV
Instruction: RPA Reaction System (for reference only)
RPA Lyophilized Kits
In addition to the previously mentioned RPA raw material enzymes, we present a varied range of kits meticulously crafted to cater to your specific requirements. All these kits are lyophilized, ensuring stability during transportation at room temperature. Delve into our exceptional selection:
Gen-1
- RPA Basic Kit (Gen-1),
- RPA RT Basic Kit (Gen-1),
- RPA Fluorescent Kit (Gen-1),
- RPA RT Fluorescent Kit (Gen-1)
Gen-2 (featuring the additional introduction of a sucrose-sucrose phosphorylase energy system for enhanced stability and reliability in the amplification process)
- RPA Basic Kit (Gen-2),
- RPA RT Basic Kit (Gen-2),
- RPA Fluorescent Kit (Gen-2),
- RPA RT Fluorescent Kit (Gen-2)
Gen-3 (featuring the additional introduction of the dUTP-UDG-UGI system to effectively prevent aerosol contamination)
- RPA Basic Kit (Gen-3),
- RPA RT Basic Kit (Gen-3),
- RPA Fluorescent Kit (Gen-3),
- RPA RT Fluorescent Kit (Gen-3)
Our kits are meticulously crafted to deliver accurate and efficient results for your research endeavors. For further information or if you're interested in any of our products, please don't hesitate to contact us. Elevate your work with our cutting-edge solutions today!
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文献和实验Ligation of DNA with T4 DNA Ligase
recombination and DNA synthesis. DNA ligase from E. coli is a polypeptide with a molecular weight of 74,000 and is NAD-dependent. T4 DNA ligase is the product of gene 30 of the T4 phage, has a molecular weight of 68,000, and is ATP-dependent. Both enzymes
Rapid Separation of Protein‐Bound DNA from Free DNA Using Nitrocellulose Filters
‐stranded, 32 P‐labeled DNA Protein sample Bovine serum albumin (BSA; optional
32P-Postlabeling Analysis of DNA Adducts
from modification of bases by endogenous or exogenous agents (e.g., oxidative damage). The procedure involves four main steps: enzymatic digestion of a DNA sample; enrichment of the adducts; radiolabeling of the adducts by T4 kinase-catalyzed transference of 32 P
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