相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保质期:
1年
- 英文名:
ZR Urine RNA Isolation Kit
- 库存:
大量
- 供应商:
简石生物
- 保存条件:
常温
HIGHLIGHTS
- Quick & Easy: Recovery of total RNA from cells, biological sediment in urine, large volume liquid samples and suitable for isolation of RNA from microvesicles. Not for cell-free isolation.
- High-Quality: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-PCR, etc.
DESCRIPTION
ZR Urine RNA Isolation Kit™ is an innovative product designed for the easy, reliable and rapid isolation of total RNA from cells in urine samples. The product enables isolation of cells from urine using a syringe (not provided) and a uniquely-designed syringe filter. Following separation, cells are lysed and total RNA stabilized using a specially formulated Urine RNA Buffer. The collected lysate can then be used immediately or at a later time following transportation and/or storage. Also, the Urine RNA Buffer is ideal for direct isolation of RNA from microvesicles that may be recovered from urine filtrates. One-step RNA isolation occurs via matrix adsorption using Zymo-Spin™ IC Columns. The RNA isolation procedure is simple and can be performed in less than 5 minutes. Use of the ZR Urine RNA Isolation Kit results in the isolation of high-quality, total RNA from urine samples that is suitable for subsequent analyses of gene expression that include RT-PCR.
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验样品裂解液 对于某些样品来说,在匀浆之后,RNA提取之前,还需要一些额外的处理步骤。对于脂肪含量高的组织,像脑组织和脂肪组织得到的裂解液,就需要通过氯仿抽提来去除脂类,从而提高RNA产量。许多植物组织中富含多酚和多糖,会降低RNA的质量和产量,用Plant RNA Isolation Aid 预处理裂解液可去除这些难以处理的成分。 5.选择最好的RNA分离方法 现有众多的RNA分离方法也许令人难以取舍。目前
。 本方法简便、快速,所提取的RNA质量较高,可直接用于cDNA合成、cDNA文库的构建以及RACE等分子生物学操作。 关键词 蜈蚣草,蕨类植物,多酚,多糖,RNA提取 An Effective Method of Total RNA Isolation from a Fern Plant — Pteris vittata L. HE Zhen-Yan XU Wen-Zhong YANG Xue-Xi MA Mi②
组织得到的裂解液,就需要通过氯仿抽提来去除脂类,从而提高RNA产量。许多植物组织中富含多酚和多糖,会降低RNA的质量和产量,用Plant RNA Isolation Aid 预处理裂解液可去除这些难以处理的成分。 5. 选择最好的RNA分离方法 现有众多的RNA分离方法也许令人难以取舍。目前最简单也是最安全的方法是柱式分离,如RNAqueous或RNAqueous-4PCR Kit。因为操作简单,所以这些步骤特别适用于同时处理多个样品。如果是处理复杂的组织,如富含核酸酶(胰腺)或脂肪(脑











