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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保质期:
1年
- 英文名:
ZR small-RNA PAGE Recovery Kit
- 库存:
大量
- 供应商:
简石生物
- 保存条件:
常温
HIGHLIGHTS
- Efficient: Recovery of ss- or ds- RNA (and DNA) fragments (17-200 nt) from up to 20% (w/v) polyacrylamide gels.
- Concentrated: Up to 10 µg sample in ≥ 6 µl elution.
- Compatible with up to 25% (w/v) polyacrylamide.
DESCRIPTION
The ZR small-RNA PAGE Recovery Kit provides an easy and efficient method for the extraction of high quality small RNAs from polyacrylamide gels (native and/or denatured). The ZR small-RNA™ PAGE Recovery Kit is a refinement of the "crush and soak" method that incorporates a unique buffer system together with Zymo-Spin column technologies for improved recovery and added convenience. The recovered RNA can be concentrated into volumes as small as 6 µl, making it ideal for many downstream enzymatic reactions and manipulations.
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文献和实验Steve HahnLast modified10/16/98Purpose: Purification of oligonucleotides (which have already been purified by reverse phase) can increase the efficiency of site directed mutagenesis from around 30% to ~75% or greater in some cases .1. Use PAGE
后的琼脂糖凝胶用与Southern 转移相同的方法将RNA 转移到硝酸纤维素滤膜上,然后与探针杂交。 1、材料:待检测的RNA 及制备好的探针。 2、设备:电泳仪,电泳槽,塑料盆,真空烤箱,放射自显影盒,X-光片,杂交袋,硝酸纤维素膜或尼龙膜。 3、试剂: (1)20×SSPE:175.3g NaCl, 88.2g 柠檬酸钠,溶于800ml 水中,用10mol/LNaOH 调pH 至7.4,定溶到1L。 (2)其他试剂:与Southern 杂交试剂类似,只是所有的试剂均应用DEPC
吸附在玻璃或塑料器皿管壁上,所有器皿一律需经硅烷化处理。 细胞内总RNA 制备方法很多,如异硫氰酸胍热苯酚法等。许多公司有现成的总RNA 提取试剂盒,可快速有效地提取到高质量的总RNA。分离的总RNA 可利用mRNA 3'末端含有多聚(A)+ 的特点,当RNA 流经oligo (dT)纤维素柱时,在高盐缓冲液作用下,mRNA 被特异的吸附在oligo(dT)纤维素上,然后逐渐降低盐浓度洗脱,在低盐溶液或蒸馏水中,mRNA 被洗下。经过两次oligo(dT)纤维素柱,可得到较纯的mRNA。纯化











