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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
1年
- 英文名:
Quick-RNA Fungal/Bacterial Miniprep Kit
- 库存:
大量
- 供应商:
简石生物
- 保存条件:
常温
HIGHLIGHTS
- High Quality: Extract intact high quality total RNA (including small/micro RNAs) from Gram-negative/postive bacteria, yeast, and fungi
- Homogenization: Ultra-high density BashingBead technology for complete disruption and sample lysis.
- Inhibitor-Free: RNA is ready for all downstream applications including sequencing, RT-PCR, microarray, etc.
DESCRIPTION
The Quick-RNA Fungal/Bacterial Microprep Kit is designed for the simple and rapid isolation of RNA from tough-to-lyse fungi, including A. fumigatus, C. albicans, N. crassa, S. cerevisiae, S. pombe, as well as Gram (+/-) bacteria, algae, and protozoa. The procedure is easy and can be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the RNA that is ideal for downstream molecular-based applications including Next-Gen sequencing, RT-PCR, microarray, hybridization, etc.
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
stained gels that the fluorescence from the RNA deriving from this miniprep is brighter than that seen when the corresponding amount of standard-prep RNA is used. This may be due to enhanced fluorescence of RNA in the presence of ATA
Yeast RNA Miniprep grow cells to mid-log phase (10-20 ml) (or take samples from time courses, 5-25 ml) spin cells, discard supernatant wash cells once with 1 ml cold RNA buffer freeze pellet in dry ice
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