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- ZYMO RESEARCH
- 美国
- R2060
- 2026年01月07日
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- 技术资料
- 保质期：
1年
- 英文名：
Direct-zol RNA Microprep Kits
- 库存：
大量
- 供应商：
简石生物
- 保存条件：
常温
HIGHLIGHTS
- Easy Handling: Bypass chloroform, phase separation and precipitation steps.
- NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
- Non-Biased: Complete RNA recovery without miRNA loss.
DESCRIPTION
The Direct-zol RNA MicroPrep Kits are RNA purification kits that provide a streamlined method for the purification of up to 10 µg (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol™ method assures unbiased recovery of small RNAs including miRNA. The procedure is easy. Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin™ Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis (including RT-PCR, transcription profiling, hybridization, sequencing etc.). The entire procedure typically takes only 7 minutes.
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文献和实验RNA FISH on cultured cells in interphase
We have adapted our protocol from commercially available random primer kits which generates specific probes with high sensitivity. The simplicity of the protocol combined with the ease of obtaining reproducible results has led to RNA FISH to becoming a routine
如果您经常遇到对RNA样品的质量拿不准的情况，为什么不试试这个？
Integrity number is a milestone for the field of gene expression. It is the first practical and reliable standard for measuring RNA quality.” ------ David Ginzinger Direct of the Genome Analysis Core Laboratory University of California, San Francisco
一样，RNeasy Protect Kits采用QIAGEN著名的硅胶膜纯化柱技术，迅速特异地吸附样品裂解液中的RNA，无需酚氯仿抽提，不用乙醇沉淀或LiCl沉淀，也不用CsCl超离，只要洗脱即可得到纯的RNA。通常情况下RNeasy的纯化技术足以除去绝大部分的DNA，而无需额外进行DNaes处理，但是对于一些对痕量DNA非常敏感的实验，用RNase-Free DNase Set（QIAGEN cat.no. 79254）可以直接在纯化柱上消化DNA残留，在随后的洗涤步骤中除去DNase，最后洗脱得到不含DNA
