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T25
IEC-6/IEC-6细胞系/IEC-6细胞株/IEC-6大鼠小肠隐窝上皮细胞
Cell line name IEC-6
Synonyms IEC 6; IEC6; Intestinal Epithelioid Cell line No. 6
Accession CVCL_0343
Resource Identification Initiative To cite this cell line use: IEC-6 (RRID:CVCL_0343)
Comments Senescence: Capable of at least 10 PDL.
Doubling time: ~20 hours (PubMed=88453); ~50 hours (DSMZ=ACC-111).
Derived from site: In situ; Small intestine; UBERON=UBERON_0002108.
Cell type: Epithelial cell of intestine; CL=CL_0002563.
Species of origin Rattus norvegicus (Rat) (NCBI Taxonomy: 10116)
Breed/subspecies: Charles River Sprague Dawley (CD(SD)); RS=RS:0000695.
Hierarchy Children:
CVCL_6973 (IEC-CF7) CVCL_A5RH (IEC6-Cdx2L1) CVCL_A5RI (IEC6-Cdx2L2)
CVCL_A5RJ (IEC6-Cdx2MT15)
Sex of cell Male
Age at sampling 18-24D
Category Finite cell line
Publications
PubMed=88453; DOI=10.1083/jcb.80.2.248; PMCID=PMC2110349
Quaroni A., Wands J.R., Trelstad R.L., Isselbacher K.J.
Epithelioid cell cultures from rat small intestine. Characterization by morphologic and immunologic criteria.
J. Cell Biol. 80:248-265(1979)
PubMed=7681632; DOI=10.1152/ajplung.1993.264.3.L229
Bijman J., Dalemans W., Kansen M., Keulemans J., Verbeek E., Hoogeveen A.T., De Jonge H., Wilke M., Dreyer D., Lecocq J.-P., Pavirani A., Scholte B.J.
Low-conductance chloride channels in IEC-6 and CF nasal cells expressing CFTR.
Am. J. Physiol. 264:L229-L235(1993)
PubMed=8552090; DOI=10.1128/mcb.16.2.619; PMCID=PMC231041
Suh E., Traber P.G.
An intestine-specific homeobox gene regulates proliferation and differentiation.
Mol. Cell. Biol. 16:619-625(1996)
PubMed=11925460; DOI=10.1093/jn/132.4.680
Thomas C., Oates P.S.
IEC-6 cells are an appropriate model of intestinal iron absorption in rats.
J. Nutr. 132:680-687(2002)
PubMed=15463957; DOI=10.1016/j.jcf.2004.05.040
Gruenert D.C., Willems M., Cassiman J.-J., Frizzell R.A.
Established cell lines used in cystic fibrosis research.
J. Cyst. Fibros. 3:191-196(2004)
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文献和实验*发表【中文论文】请标注:由上海酶研生物科技有限公司提供;
*发表【英文论文】请标注:From Shanghai EK-Bioscience Biotechnology Co., Ltd.
离子交换层析(Ion Exchange Chromatography ,IEC)
相关专题 简介 离子交换层析 (Ion Exchange Chromatography 简称为IEC)是以离子交换剂为固定相,依据流动相中的组分离子与交换剂上的平衡离子进行可逆交换时的结合力大小的差别而进行分离的一种层析方法。1848年,Thompson 等人在研究土壤碱性物质交换过程中发现离子交换现象。本世纪40年代,出现了具有稳定交换特性的聚苯乙烯离子交换树脂。50年代,离子交换层析进入生物化学领域,应用于氨基酸的分析
Separation, Identification, and Profiling of Membrane Proteins by GFC/IEC/SDS-PAGE and MALDI TOF MS
detergent n -dodecyl-β-d-maltoside, proteins can be separated by ion-exchange chromatography (IEC) and further resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). An additional separation step by gel filtration (GF) before IEC
1、打开离心机电源开关,进入待机状态。 2、选择合适的转头:877转头配套的离心管为200ml专用管,875转头配套离心管为40ml专用管,离心时离心管所盛液体不能超过总容量的2/3,否则液体易于溢出;使用前后应注意转头内有无漏出液体残余,应使之保持干燥。转换转头时应注意使离心机转轴和转头的卡口卡牢。 3、离心管平衡误差应在0.1克以内。 4、选择离心参数: 按温度设置按钮,再用数字键设置离心温度,回车确定。 按速度设置按钮,可在RPM/RCF设置档之间切换,用数字键设置离心
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