- ￥1480
- EK-Bioscience
- CCC-Y1010
- 2026年01月08日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 规格：
T25
293T细胞293T人胚肾细胞
Cell line name HEK293T
Synonyms Hek293T; HEK-293T; HEK 293T; HEK-293-T; HEK 293 T; 293-T; 293 T; 293T; Human Embryonic Kidney 293T; 293tsA1609neo
Accession CVCL_0063
Resource Identification Initiative To cite this cell line use: HEK293T (RRID:CVCL_0063)
Comments Part of: ENCODE project common cell types; tier 3.
Part of: MD Anderson Cell Lines Project.
Virology: Highly susceptible to infection by Zika virus (ZIKV) (PubMed=29468137).
Doubling time: ~24-30 hours (DSMZ=ACC-635).
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
Omics: Deep proteome analysis.
Omics: Genome sequenced.
Omics: miRNA expression profiling.
Omics: Mitochondrial proteome analysis.
Omics: Protein expression by reverse-phase protein arrays.
Omics: Transcriptome analysis by RNAseq.
Misspelling: HECK293T; Note=Occasionally.
Misspelling: HEK 239T; Note=Occasionally.
Misspelling: HEK239T; Note=Occasionally.
Misspelling: HEK-393T; Note=Occasionally.
Misspelling: HEK393T; Note=Occasionally.
Misspelling: 239T; Note=Occasionally.
Misspelling: 393T; Note=Occasionally.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
PubMed=3031469; DOI=10.1128/mcb.7.1.379-387.1987; PMCID=PMC365079
DuBridge R.B., Tang P., Hsia H.-C., Leong P.-M., Miller J.H., Calos M.P.
Analysis of mutation in human cells by using an Epstein-Barr virus shuttle system.
Mol. Cell. Biol. 7:379-387(1987)
PubMed=15900046; DOI=10.1093/jnci/dji133
Mashima T., Oh-hara T., Sato S., Mochizuki M., Sugimoto Y., Yamazaki K., Hamada J.-i., Tada M., Moriuchi T., Ishikawa Y., Kato Y., Tomoda H., Yamori T., Tsuruo T.
p53-defective tumors with a functional apoptosome-mediated pathway: a new therapeutic target.
J. Natl. Cancer Inst. 97:765-777(2005)
PubMed=25182477; DOI=10.1038/ncomms5767; PMCID=PMC4166678
Lin Y.-C., Boone M., Meuris L., Lemmens I., Van Roy N., Soete A., Reumers J., Moisse M., Plaisance S., Drmanac R., Chen J., Speleman F., Lambrechts D., Van de Peer Y., Tavernier J., Callewaert N.
Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations.
Nat. Commun. 5:4767.1-4767.12(2014)
PubMed=26694163; DOI=10.1371/journal.pone.0144924; PMCID=PMC4692263
Janiszewska J., Szaumkessel M., Kostrzewska-Poczekaj M., Bednarek K., Paczkowska J., Jackowska J., Grenman R., Szyfter K., Wierzbicka M., Giefing M., Jarmuz-Szymczak M.
Global miRNA expression profiling identifies miR-1290 as novel potential oncomiR in laryngeal carcinoma.
PLoS ONE 10:E0144924-E0144924(2015)
PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005; PMCID=PMC5501076
Li J., Zhao W., Akbani R., Liu W.-B., Ju Z.-L., Ling S.-Y., Vellano C.P., Roebuck P., Yu Q.-H., Eterovic A.K., Byers L.A., Davies M.A., Deng W.-L., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y.-L., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)
PubMed=28601559; DOI=10.1016/j.cels.2017.05.009; PMCID=PMC5493283
Bekker-Jensen D.B., Kelstrup C.D., Batth T.S., Larsen S.C., Haldrup C., Bramsen J.B., Sorensen K.D., Hoyer S., Orntoft T.F., Lindbjerg Andersen C., Nielsen M.L., Olsen J.V.
An optimized shotgun strategy for the rapid generation of comprehensive human proteomes.
Cell Syst. 4:587-599.e4(2017)
DOI=10.13005/bpj/1414
Yuan J., Xu W.W.-Y., Jiang S.Y.-L., Yu H.X.-Y., Poon H.-F.
The scattered twelve tribes of HEK293.
Biomed. Pharmacol. J. 11:621-623(2018)
PubMed=29468137; DOI=10.5501/wjv.v7.i1.10; PMCID=PMC5807893
Himmelsbach K., Hildt E.
Identification of various cell culture models for the study of Zika virus.
World J. Virol. 7:10-20(2018)
PubMed=34800366; DOI=10.1016/j.cmet.2021.11.001; PMCID=PMC8664129
Morgenstern M., Peikert C.D., Lubbert P., Suppanz I., Klemm C., Alka O., Steiert C., Naumenko N., Schendzielorz A., Melchionda L., Muhlhauser W.W.D., Knapp B., Busch J.D., Stiller S.B., Dannenmaier S., Lindau C., Licheva M., Eickhorst C., Galbusera R., Zerbes R.M., Ryan M.T., Kraft C., Kozjak-Pavlovic V., Drepper F., Dennerlein S., Oeljeklaus S., Pfanner N., Wiedemann N., Warscheid B.
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
Cell Metab. 33:2464-2483.e18(2021)
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验*发表【中文论文】请标注：由上海酶研生物科技有限公司提供；
*发表【英文论文】请标注：From Shanghai EK-Bioscience Biotechnology Co., Ltd.
关于293/293T细胞1、种属都为人，不是鼠。2、来源：293细胞是转染腺病毒E1A基因的人肾上皮细胞系，293T细胞由293细胞派生，同时表达SV40大T抗原，含有SV40复制起始点与启动子区的质粒可以复制。用Ca3(PO4)2转染效率可高达50%。蛋白表达水平高，转染后2-3天用碱性磷酸酶分析可较容易地检测到表达的蛋白。瞬时转染293T细胞是过表达蛋白并获得细胞内及细胞外（分泌的或膜）蛋白的便捷方式。3、形态为上皮细胞，能致瘤。4、染色体核型：亚三倍体人细胞系。染色体众数为64，30
包装细胞293T细胞的培养一、293T细胞的冻存1. 随着传代的次数增加，293T细胞会出现生长状态下降，出现突变等。所以要在细胞购进时就进行大量冻存，以保证实验的稳定性和持续性。2. 在细胞对数生长期进行冻存，增加细胞复苏成活率。3. 倒去细胞上清液，加入D-Hank's液洗去残留的培养基。4. 加入0.25%的胰酶，消化10-20s后倒去。5. 镜下观察细胞变圆，细胞间间隙加大时，加入新鲜培养基吹打混匀。6. 细胞计数。7. 将细胞离心，1000rpm，2min。8.根据计数结果加入
相关专题 293细胞 关于293/293T细胞 1、种属都为人，不是鼠。 2、来源：293细胞是转染腺病毒E1A基因的人肾上皮细胞系，293T细胞由293细胞派生，同时表达SV40大T抗原 ，含有SV40复制起始点与启动子区的质粒可以复制。用Ca3(PO4)2转染效率可高达50%。蛋白表达水平高，转染后2-3天用碱性磷酸酶分析可较容易地检测到表达的蛋白。瞬时转染293T细胞是过表达蛋白并获得细胞内及细胞外(分泌
技术资料