- ￥2310 - 25190
- Sino Biological
- 北京
- 50231-M08H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Mouse CD73 / NT5E Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
10 µg/50 µg/20 µg/1 mg
| 规格： | 10 µg | 产品价格： | ￥2310.0 |
|---|---|---|---|
| 规格： | 50 µg | 产品价格： | ￥2310.0 |
| 规格： | 20 µg | 产品价格： | ￥3870.0 |
| 规格： | 1 mg | 产品价格： | ￥25190.0 |
蛋白名称：Mouse CD73 / NT5E Protein (His Tag)
蛋白构建：A DNA sequence encoding the mouse NT5E (NP_035981.1) without the propeptide, corresponding to the amino acid (Met 1-Lys 549) was expressed, with a C-terminal polyhistidine tag.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996). The specific activity is >10,000 pmol/min/µg.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Trp 29
蛋白分子量：The secreted recombinant mouse NT5E consists of 532 amino acids and has a calculated molecular mass of 59.4 kDa which is also detected in SDS-PAGE under reducing conditions.
蛋白NP号：NP_035981.1
蛋白氨基酸序列：Met1-Lys549
蛋白标签：C-His
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文献和实验in the expressed sequence tag or unannotated DNA databases and were novel proteins of unknown function. Since that time, we have been using various molecular biological approaches to determine the functions of some of these proteins
mutations at phenylalanine in a putative PP-1C (the catalytic subunit) binding site motif(K/R)(X)(I/V)XF was unable to rescue Hxk2p dephosphorylation in intact yeast or restore Hxk2p phosphatase activity. These results demonstrate that Reg1p targets PP-1C
。当某一个标签的使用，一是能构成表位利于纯化和检测；二是构成独特的结构特征（结合配体）利于纯化。组氨酸残基侧链与固态的镍有强烈的吸引力，可用于固定化金属螯合层析(IMAC)，对重组蛋白进行分离纯化。使用His-tag有下面优点：1.标签的分子量小，只有~0.84KD，而GST和蛋白A分别为~26KD和~30KD，一般不影响目标蛋白的功能；2.His标签融合蛋白可以在非离子型表面活性剂存在的条件下或变性条件下纯化，前者在纯化疏水性强的蛋白得到应用，后者在纯化包涵体蛋白时特别有用，用高浓度的变性剂溶解
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