- ￥1920 - 15050
- Sino Biological
- 北京
- 11524-H02H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human CD28 Protein (ECD,Fc Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
100 µg/200 µg/1 mg
| 规格： | 100 µg | 产品价格： | ￥1920.0 |
|---|---|---|---|
| 规格： | 200 µg | 产品价格： | ￥2570.0 |
| 规格： | 1 mg | 产品价格： | ￥15050.0 |
蛋白名称：Human CD28 Protein (ECD,Fc Tag)
蛋白构建：A DNA sequence encoding the human CD28 isoform 1 (P10747-1) extracellular domain (Met 1-Pro 152) was was fused with the Fc region of human IgG1 at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested).
蛋白活性：1.Loaded Recombinant Human B7-1 Protein, His Tag(Cat. No. 10698-H08H) on His1K Biosensor, can bind Recombinant Human CD28 Protein, hFc Tag (Cat. No. 11524-H02H) with an affinity constant of 0.46 µM as determined in BLI assay (Sartorius Octet RED384) (QC tested). 2.Loaded Recombinant Human CD86 Protein, His Tag(Cat. No. 10699-H08H) on His1K Biosensor, can bind Recombinant Human CD28 Protein, hFc Tag (Cat. No. 11524-H02H) with an affinity constant of 2.72 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Asn 19
蛋白分子量：The recombinant human CD28/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 375 amino acids and predictes a molecular mass of 42 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhCD28/Fc monomer is approximately 55-65 kDa due to glycosylation. The molecular weight of this protein is around 112.6 kDa verified by SEC-MALS (Routinely tested).
蛋白NP号：P10747-1
蛋白氨基酸序列：Met1-Pro152
蛋白标签：C-human IgG1-Fc
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文献和实验【原创】Protein A Sepharose 之进化篇。。。
洗脱条件比较剧烈，最后收集的蛋白质很有可能变性，或者是复性困难。 这种洗脱条件剧烈的Protein A 柱结合的重组蛋白质A一般具有5个串联结构域：E、D、A、B、C。这种重组蛋白A虽然除去了不重要的非结合域，仅由5个结构域组成，每个域均可以和IgG的Fc段结合，但不同的域结合强度略有差异。因此洗脱条件不均一，而且经常需要较低的pH值。如果减少串联结构域的个数，并且采取同型结构域串联，就可以避免不同结构域与抗体Fc 段亲和性的差异，洗脱条件会温和而均一。为此，我们可以做一个试验，比较一下
Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
RPMI Medium 1640 with 2 mM L-Glutamin, 10% FCS/FBS and 100 U/ml penicillin/streptomycin can be used, or an equivalent culture medium. Heat inactivated Fetal Calf Serum (FCS). Recombinant mouse IL-2 (human IL-2 can also be used).
Transient Production of scFv-Fc Fusion Proteins in Mammalian Cells
Single chain Fv (scFv) antibody fragments fused to the human immunoglobulin G (IgG) Fc moiety obtain IgG-like properties, but nevertheless they are still encoded by a single gene. As transient production of scFv-Fc proteins in mammalians
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