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文献和实验Studies of the Ubiquitin Proteasome System
antibody for the protein of interest and isotype‐matched control antibody, e.g., irrelevant or preimmune serum, purified Tris‐buffered saline/Tween 20 (TBST; see recipe ), 4°C Cells growing in culture
免疫组化技术-Immunohistochemical Protocols
Incubation in affinity purified indocarbocyanine (Cy3, viewed in red field, 1:1000 in BSA diluent) or fluorescein isothiocyanate (FITC, viewed in green field, 1:100 in BSA diluent) fluorochrome conjugated secondary antibodies (F[ab']2 fragments
HIGH RESOLUTION GENETIC FOOTPRINTING
3 below for design of alternative substrates). 2.) MLV integrase enzyme. Moloney Murine Leukemia Virus Integrase (MLV IN) is purified as a fusion to glutathione S-transferase. A single-step glutathione agarose affinity purification generates enzyme
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