AHK2 | Histidine kinase 2,(Hos
t: Rabbit),(Purity: Affinity purified serum in PBS, pH 7.4)- ￥6900
- Cedarlane
- CLAS12-2113
- 2025年07月16日
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- 库存：
999
- 规格：
50 µg
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文献和实验v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
-1 and sFRP-2. Alternatively, sFRP derivatives taggedwith poly-histidine at their carboxyl termini are functional and can be readily isolated by chelating chromatography.Once purified, the proteins are stable indefinitely if stored frozen and they tolerate
by the presence of detergent. If purified Wnt3A isdiluted into an aqueous buffer, either detergent or 10% FBSshould be included. In fact, dilution of purified Wnt into abuffer lacking either detergent or serum will lead to precipitationand loss of activity
Studies of the Ubiquitin Proteasome System
antibody for the protein of interest and isotype‐matched control antibody, e.g., irrelevant or preimmune serum, purified Tris‐buffered saline/Tween 20 (TBST; see recipe ), 4°C Cells growing in culture
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