PTTG2基因敲除质粒

pLenti-PTTG2-sgRNA (PTTG2基因敲除质粒)是一种在动物细胞中可以同时表达Cas9、目的基因的sgRNA和puromycin抗性基因的质粒。用于在动物细胞中直接基于CRISPR/Cas9技术敲除目的基因，或者通过包装慢病毒后基于CRISPR/Cas9技术敲除目的基因。本质粒中sgRNA的有效性已经通过T7EI法的验证。

本质粒在细菌中为Amp抗性，全长约13,000bp。本质粒的关键图谱信息请参考图1。本质粒可直接转染细胞用于目的基因的CRISPR/Cas9敲除，以及通过puromycin筛选稳定细胞株；也可以与pMDLg、Rev及VSV-g共转HEK293T细胞进行重组慢病毒(lentivirus)的包装，然后再用于感染细胞或组织并进行目的基因的CRISPR/Cas9敲除。

图1. 表达sgRNA、Cas9和puromycin抗性的pLenti-sgRNA质粒关键图谱信息。

本质粒中的sgRNA基于碧云天研发的CRISPR/Cas9 sgRNA快速筛选和验证体系获得，sgRNA的有效性已经通过T7EI法验证。

本质粒用于实验时，建议同时选购无任何靶向的对照质粒pLenti-Control-sgRNA (L00011)或靶向GFP的对照质粒pLenti-GFP-sgRNA (L00013)。

碧云天同时提供基于CRISPR/Cas9技术的PTTG2基因敲除的质粒(L03885 pLenti-PTTG2-sgRNA)、慢病毒(L03886 PTTG2 Knockout Lentivirus)、HEK293T细胞(L03887 PTTG2 Knockout HEK293T Cells)、HEK293T敲除细胞的RIPA裂解液(L03888 PTTG2 Knockout HEK293T RIPA Lysate)、HEK293T敲除细胞的Trizol裂解液(L03889 PTTG2 Knockout HEK293T Trizol Lysate)等产品，具体请在碧云天网站查询或在本产品网页点击相应产品。

PTTG2基因的基本信息如下：

Species	Gene Symbol	Gene ID	GenBank Accession	Transcript
Human	PTTG2	10744	BC069114	NM_006607

 

About the gene
Official Symbol	PTTG2
Previous Symbol	-
Official Full Name	pituitary tumor-transforming 2
Synonyms	Q9NZH5.2
Location	4p14
Gene Type	protein_coding
Uniprot ID	Q9NZH5
Pathway/Library	Oncogenes Library
Gene Summary	Securin is a regulatory protein that contributes to mitotic checkpoint control, p53 activity, DNA repair, and cell migration. The securin protein prevents premature separation of sister chromatids by inhibiting the protease separase, which cleaves the cohesin complex at the onset of anaphase to allow for sister chromatid segregation. In mouse oocytes, securin regulates cyclin B stability and G2/M transition. Securin negatively affects the transcriptional activity of p53, preventing p53-regulated apoptosis. Research studies indicate that securin plays a role in microtubule nucleation, cell polarization, and cell migration. Additional research indicates that securin expression may be useful as a biomarker for human breast cancer. The pituitary tumor-transforming gene 1 (PTTG1) encodes for securin protein, while the highly homologous securin 2 protein is transcribed from the related PTTG2 gene. Securin 2 (PTTG2) protein lacks the ability to inhibit separase activity and may function primarily in cell adhesion and migration.