- ￥4500
- Sino Biological
- 北京
- 40592-V08H85
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant SARS-CoV-2 Spike RBD(K417N,E484K,N501Y)-His Recombinant Protein
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
100.00 µg
纯度> 90 % as determined by SDS-PAGE.
内毒素< 1.0 EU per μg protein as determined by the LAL method.
生物活性Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag)(Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD(K417N, E484K, N501Y)-His (40592-V08H85), the EC50 of SARS-CoV-2 (2019-nCoV) Spike RBD(K417N, E484K, N501Y)-His (40592-V08H85) is 5-40 ng/mL.
蛋白构建A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike RBD(K417N, E484K, N501Y)-His Recombinant Protein (YP_009724390.1) (Arg319-Phe541(K417N, E484K, N501Y)) was expressed with a polyhistidine tag at the C-terminus.
表达宿主HEK293 Cells
种属SARS-CoV-2
预测 N 端Arg 319
分子量The recombinant SARS-CoV-2 (2019-nCoV) Spike S1(Arg319-Phe541(K417N, E484K, N501Y))-His Recombinant Protein consists of 234 amino acids and predicts a molecular mass of 26.6 kDa. As a result of glycosylation, it migrates as an approximately 35 kDa band in SDS-PAGE under reducing conditions.
缓冲液Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
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文献和实验2, Chen C, et al. Potent and Broad Neutralization of SARS-CoV-2 Variants of Concern (VOCs) including Omicron Sub-lineages BA.1 and BA.2 by Biparatopic Human VH Domains.iScience, PubMed ID: 35875685
3, Park S, et al. An ancestral SARS-CoV-2 vaccine induces anti-Omicron variants antibodies by hypermutation.Nature communications, PubMed ID: 38643233
日前，北京大学生命科学学院魏文胜课题组在 Cell 杂志上在线发表题为“Circular RNA Vaccines against SARS-CoV-2 and Emerging Variants”的研究论文。 魏文胜团队首先建立了体外高效制备高纯度环状 RNA 的技术平台，针对新型冠状病毒及其变异株，设计了编码新冠病毒刺突蛋白（Spike）受体结构域（RBD）的环状 RNA 疫苗。 该项研究中制备的针对新冠病毒德尔塔变异株的环状 RNA 疫苗（circRNARBD
利用 Millicell 培养小室培养皮肤及肺类器官的实验方案
2011. Alternative (non-animal) methods for cosmetics testing: current status and future prospects?2010. Arch Toxicol. 85(5):367-485. http://dx.doi.org/10.1007/s00204-011-0693-2 2.Abd E, Yousuf S, Pastore M, Telaprolu K, Mohammed Y, Namjoshi S, Grice J,
) 。 ( 4 ) 由于胰蛋白酶的独特性，使它成为在蛋白质胶内酶解之后用于 MS 鉴定的合适内切酶： a. 分子质量相对低的内切酶（约 24 kDa) ，用水化缓冲液溶解之后很容易渗透进入冷冻干燥的含蛋白质点的胶粒中。 b. 优良的酶切特性（酶切 K 和 R 的 C 端，如果下一个氨基酸是 P 则不进行酶切，如果 D/E 氨基酸的支链酸性端出现在旁边，则它的酶切效率降低，由于支链的空间效应，如果大量 F/W/Y 氨基酸出现在旁边，则它的酶切效率降低）。商业猪胰蛋白酶用 N-甲苯磺酰-L-苯基丙氨酸氯
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