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- 详细信息
- 文献和实验
- 技术资料
- 库存：
现货
- 供应商：
上海经科化学科技有限公司
- 规格：
50μg/1mg/500μg/10μg
| 规格： | 50μg | 产品价格： | ￥1280.0 |
|---|---|---|---|
| 规格： | 1mg | 产品价格： | ￥2580.0 |
| 规格： | 500μg | 产品价格： | ￥1880.0 |
| 规格： | 10μg | 产品价格： | ￥600.0 |
产品信息
| 别名 | Somatamedin C, IGF-IA |
| 物种 | Human |
| 表达宿主 | E.coli |
| 序列信息 | MGPETLCGAELVDALQFVCGDRGFYFNKPTGYG,S.SSRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPAKSA with polyhistidine tag at the C-terminus. |
| 检索号 | P05019.1 |
| 分子量 | 8.59 kDa |
| 表观分子量 | 8-10 kDa |
| 标签 | His-tag at the C-terminus |
| 生物活性 | Measure by its ability to induce MCF-7 cells proliferation. The ED50 for this effect is 0.9-3.1 ng/mL. The specific activity of recombinant human IGF-I is approximately >1.2 x 10^3 IU/mg. |
产品特性
| 纯度 | >95% as determined by SDS-PAGE. Ni-NTA chromatography. |
| 内毒素 | <0.01 EU per 1 μg of the protein by the LAL method. |
| 保存 | Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution,store at 2-8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g.0.1% BSA,10%FBS,5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months. |
| 运输 | Ambient temperature or ice pack. |
| 制剂 | The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4. |
| 复融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
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