Recombinant Human IGF-I R (C-6

Recombinant Human IGF-I R (C-6His) (C08K)

 

产品说明(Description)

Recombinant Human Insulin-like Growth Factor 1 Receptor is produced by our Mammalian expression system and the target gene encoding Glu31­Asn932  is expressed with a 6His tag at the C-terminus.

Accession #: P08069

Known as: CD221 antigen; CD221; IGF1R; IGF-1R; IGF-I R; IGF-I receptor; IGFIR; IGF-IR; IGFR; insulin-like growth factor 1 receptor; JTK13

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of PBS, 1mM EDTA , 0.5% Tween-20, 5% Trehalose, pH 7.4.

 

质量控制(Quality Control)

 

Purity:	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin:	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

 

背景(Background)

The insulin-like growth factor-1 receptor (IGF1R) is a transmembrane tyrosine kinase involved in several biological processes including cell proliferation, differentiation, DNA repair, and cell survival. This a disulfide-linked heterotetrameric transmembrane protein consisting of two α and two β subunits, and among which, the α subunit is extracellular while the β subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF-I receptor is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and II have been shown to be mediated via IGF-I R. IGF1R is an important signaling molecule in cancer cells and plays an essential role in the establishment and maintenance of the transformed phenotype. Inhibition of IGF1R signaling thus appears to be a promising strategy to interfere with the growth and survival of cancer cells, is now an attractive anti-cancer treatment target.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY