Somatamedin C, IGF-IA, 重组蛋白,细胞

产品概述

产品名称：Somatamedin C / IGF‑IA（重组人胰岛素样生长因子Ⅰ，His‑标签）
英文名称：Human IGF‑I Protein, His tag (Animal‑Free)
货号：PRP1933
别名：Somatamedin C, IGF‑IA

本品为来源于人胰岛素样生长因子Ⅰ（Gly49‑Ala118）区域的重组蛋白，采用大肠杆菌表达系统生产，适用于细胞培养中的细胞因子研究。产品以无菌PBS（pH 7.4）缓冲的冻干粉形式提供，满足细胞增殖、代谢调控等多种体外实验需求。

关键优势

• 高纯度：经SDS‑PAGE检测，纯度>95%。
• 低内毒素：采用LAL法检测，每µg蛋白内毒素含量<0.01 EU，适合对内毒素敏感的细胞系。
• 功能验证：以诱导MCF‑7细胞增殖的ED50为0.9‑3.1 ng/mL，生物活性约为1.2 × 10³ IU/mg。
• 动物源‑自由：His‑标签蛋白生产过程中不使用动物源材料，降低交叉污染风险。

技术参数

项目	内容
分子量	约11 kDa
氨基酸序列来源	人胰岛素样生长因子Ⅰ（Gly49‑Ala118）
表达宿主	大肠杆菌
纯度检测	SDS‑PAGE >95%
活性指标	ED50 (MCF‑7) 0.9‑3.1 ng/mL；比活力 1.2 × 10³ IU/mg
内毒素水平	<0.01 EU/µg
产品形态	冻干粉（无菌PBS pH 7.4 缓冲）
包装规格	100 µg / 500 µg / 1 mg / 10 mg

使用说明与注意事项

复溶

使用随包装提供的Reconstitution Buffer（无菌PBS），将冻干粉复溶至不低于 100 µg/mL。建议在复溶后进行一次离心（< 12000 g，5 min），去除可能的沉淀。

稀释与应用

依据实验需求，可在含有载体蛋白或稳定剂的缓冲液中进一步稀释，例如 0.1% BSA、10% FBS 或 5% HSA，以提高蛋白的稳定性。推荐的工作浓度范围请参考各自实验方案。

保存

• 冻干状态：-20 °C，保质期 1 年。
• 复溶后（2‑8 °C）：最多保存 1 周。
• 含载体蛋白/稳定剂的二次稀释液：-20 °C 或 -80 °C 保存 3‑6 个月，避免反复冻融。

运输

采用冰袋（蓝冰）冷链运输，确保全程低温。

合规与安全

本产品为科研级试剂，仅限于体外实验使用，不得用于人类或临床诊断。

使用本品不构成对任何专利或许可证的侵权建议。若因使用本产品导致的专利纠纷或其他违规行为，供应商不承担责任。

订购信息

规格	单价 (人民币)	库存状态
100 µg	588	现货（次日发货）
500 µg	1498	请咨询
1 mg	1998	请咨询
10 mg	待报价	请咨询

产品背景简述

胰岛素样生长因子Ⅰ（IGF‑I）是体内关键的生长调节因子，约 7.79 kDa，含 71 个氨基酸残基。它在肝脏、脂肪组织、子宫内膜基质细胞及其他间质细胞中高表达。IGF‑I 通过与 IGF‑I 受体结合，参与蛋白质合成、垂体促生长激素释放、糖原代谢、DNA 合成及葡萄糖摄取等多条信号通路，是细胞增殖与代谢研究的重要工具。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Product Name: Somatamedin C / IGF‑IA (Recombinant Human Insulin-like Growth Factor I, His-Tag)
English Name: Human IGF‑I Protein, His tag (Animal-Free)
Catalog Number: PRP1933
Alias: Somatamedin C, IGF‑IA

This product is a recombinant protein derived from the human Insulin-like Growth Factor I (Gly49-Ala118) region, produced using an E. coli expression system. It is suitable for cytokine research in cell culture. The product is supplied as a lyophilized powder sterilely buffered in PBS (pH 7.4), meeting various in vitro experimental needs such as cell proliferation and metabolic regulation.

Key Advantages

• High Purity: Purity >95% as determined by SDS-PAGE.
• Low Endotoxin: Endotoxin level <0.01 EU/µg protein as measured by LAL assay, suitable for endotoxin-sensitive cell lines.
• Functionally Validated: ED50 for inducing MCF-7 cell proliferation is 0.9-3.1 ng/mL; specific activity is approximately 1.2 × 10³ IU/mg.
• Animal-Free: The His-tagged protein is produced without animal-derived materials, reducing the risk of cross-contamination.

Specifications

Item	Content
Molecular Weight	Approximately 11 kDa
Amino Acid Sequence Source	Human Insulin-like Growth Factor I (Gly49-Ala118)
Expression Host	E. coli
Purity	>95% by SDS-PAGE
Activity	ED50 (MCF-7) 0.9-3.1 ng/mL; Specific Activity 1.2 × 10³ IU/mg
Endotoxin Level	<0.01 EU/µg
Form	Lyophilized powder (sterile PBS, pH 7.4)
Package Size	100 µg / 500 µg / 1 mg / 10 mg

Usage & Precautions

Reconstitution

Reconstitute the lyophilized powder to a concentration not less than 100 µg/mL using the provided Reconstitution Buffer (sterile PBS). A brief centrifugation (< 12000 g, 5 min) is recommended after reconstitution to remove any potential insoluble particulates.

Dilution & Application

For experimental use, further dilution can be performed in buffers containing carrier proteins or stabilizers, such as 0.1% BSA, 10% FBS, or 5% HSA, to enhance protein stability. Please refer to specific experimental protocols for recommended working concentration ranges.

Storage

• Lyophilized State: -20 °C, stable for 1 year.
• Reconstituted (2‑8 °C): Stable for up to 1 week.
• Secondary Dilutions (with carrier/stabilizer): Store at -20 °C or -80 °C for 3‑6 months; avoid repeated freeze-thaw cycles.

Shipping

Shipped with ice packs (blue ice) to ensure a consistent cold chain.

Compliance & Safety

This product is a Research Grade reagent intended for in vitro use only. It is not for human or diagnostic use.

Use of this product does not imply a license or freedom to practice under any patent. The supplier is not responsible for patent infringement or other violations arising from the use of this product.

Ordering Information

Package Size	Unit Price (RMB)	Stock Status
100 µg	588	In Stock (Ships Next Day)
500 µg	1498	Please Inquire
1 mg	1998	Please Inquire
10 mg	Price on Request	Please Inquire

Background

Insulin-like Growth Factor I (IGF‑I) is a key growth-regulating factor in the body, approximately 7.79 kDa, consisting of 71 amino acid residues. It is highly expressed in the liver, adipose tissue, endometrial stromal cells, and other mesenchymal cells. IGF‑I binds to the IGF‑I receptor and participates in multiple signaling pathways, including protein synthesis, pituitary growth hormone release, glycogen metabolism, DNA synthesis, and glucose uptake, making it an important tool for studying cell proliferation and metabolism.

Note: The above information is subject to change due to product optimization and upgrades. Please consult Yakeyin Company for specific details.