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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Influenza A H5N1 Nucleoprotein / NP Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
100 µg
蛋白名称：Influenza A H5N1 (A/Texas/37/2024) Nucleoprotein / NP Protein (His Tag) (HPLC-verified)
蛋白构建：A DNA sequence encoding the Influenza A virus ((A/Texas/37/2024) (H5N1)) Nucleoprotein / NP (EPI3171489) (Met1-Asn498) was expressed with a polyhistidine tag at the N-terminus.
表达宿主：HEK293 Cells
蛋白纯度：≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Met
蛋白分子量：The recombinant Influenza A virus (A/Texas/37/2024 (H5N1))Nucleoprotein / NP comprises 505 amino acids and has a predicted molecular mass of 57.28 kDa.
蛋白NP号：EPI3171489
蛋白氨基酸序列：Met1-Asn498
蛋白标签：N-His
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文献和实验The Mouse as Model System to Study Host‐Pathogen Interactions in Influenza A Infections
., Watanabe, S., Shinya, K., Nguyen, T., Lien, P.S., Le, Q.M., and Kawaoka, Y. 2007. Growth of H5N1 influenza A viruses in the upper respiratory tracts of mice. PLoS Pathog. 3:1374‐1379.
Assessing Antigen-Specific CD8+ CTL Responses in Humans
fragments and synthetic peptides to localize epitopes. It was subsequently established that target cells that had been incubated with short synthetic peptides corresponding to the sequence of the influenza virus nucleoprotein (NP) could be specifically
液，比如 RIPA，虽然裂解剧烈，但是无法维持蛋白天然构象，会破坏互作蛋白对。 如果自配，建议采用温和系统，比如 NP40/Triton 0.1%-1%；SDS ≤0.1%，盐离子≤150 mM 记得要加蛋白酶和/或磷酸酶抑制剂 结合液---利于蛋白之间的相互结合，要考虑孵育（结合）顺序 如果抗体和 beads 先孵育，建议 选择 pH 8.2（更常用）或者 pH 5.0 如果选用纯抗原作为诱饵蛋白 X 去拉裂解液中的靶蛋白 Y，抗体和抗原的结合 buffer 选择中性PBS/TBS 如果诱饵蛋白 X
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