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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human CCR8 Protein (Nanodisc, Flag-Strep & His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
20 µg/100 µg/500 µg
| 规格: | 20 µg | 产品价格: | ¥11980.0 |
|---|---|---|---|
| 规格: | 100 µg | 产品价格: | ¥41980.0 |
| 规格: | 500 µg | 产品价格: | ¥83980.0 |
蛋白名称:Human CCR8 Protein (Nanodisc, Flag-Strep & His Tag)
蛋白构建:A DNA sequence encoding the Human CCR8 (P51685) (Met1-Cys228 & Asn230-Ser318, thermostabilizing mutations) (TM domains 1-7) was expressed and linked by a Fusion Protein, with a Flag tag followed by a Strep Tag at the N-terminus, a polyhistidine tag at the C-terminus. 〈br/〉Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment.
表达宿主:HEK293 Cells
蛋白纯度:≥ 85 % as determined by SDS-PAGE.
蛋白活性:1. Immobilized Recombinant Human CCR8-Nanodisc Protein (Cat:11450-H91H-NA) at 5 μg/mL (100 μL/well) can bind Anti- Human CCR8 Monoclonal Antibody1, Human IgG1, the EC50 is 1-5 ng/mL (QC tested). 2. Immobilized Recombinant Human CCR8-Nanodisc Protein (Cat:11450-H91H-NA) at 5 μg/mL (100 μL/well) can bind Anti- Human CCR8 Monoclonal Antibody2, Human IgG1, the EC50 is 3-10 ng/mL (Routinely tested). 3. Immobilized Recombinant Human CCR8-Nanodisc Protein (Cat:11450-H91H-NA) at 5 μg/mL (100 μL/well) can bind Anti- Human CCR8 Monoclonal Antibody3, Human IgG1, the EC50 is 3-10 ng/mL (Routinely tested).
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Met 1
蛋白分子量:The recombinant Human CCR8 protein predicts a molecular mass of 46.51 kDa. The recombinant protein migrates as an approximately 96.9 kDa (dimer) and 44 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP号:P51685
蛋白氨基酸序列:Met1-Cys228 & Asn230-Ser318
蛋白标签:N-Flag-Strep & C-His
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文献和实验北京义翘神州生物技术有限公司(Sino Biological Inc.)--致力于为全球生命科学研究人员提供高质量的重组蛋白、抗体等科研工具试剂。 • 国际一流的团队:4名中央"千人计划",300人科研团队 • 一流的技术平台:蛋白表达大规模生产,鼠单抗与兔单抗技术 • 国内最大蛋白、抗体库:2000多种蛋白,1500多种抗体 • 抗体检测限比国际知名品牌抗体低10~160倍 • 3000多家全球客户:哈佛医学院、斯坦
: 1、蛋白质电泳实验技术.pdf 2、蛋白质纯化与鉴定实验指南.pdf 3、蛋白质技术手册-汪家政.pdf 4、蛋白质分子基础(第二版).pdf 5、常见重组蛋白纯化(chromatography).pdf 6、分离纯化工艺原理.pdf 7、蛋白质连接技术.pdf 8、Protein Structure, Stability, and Folding 蛋白质结构、稳定性和折叠.pdf 9、蛋白质结构分析:制备、鉴定与微量测序.pdf 10、蛋白质的结构预测与分子设计.pdf 11
肝刺激因子对人肝癌细胞BEL-7402 p21ras表达的影响
hepatocyte mitogen)。相信以HSS重组蛋白和制得的相应抗体进行示踪, 将有助于深入了解HSS跨膜信号传导的分子生物学机制, 揭示HSS作用之本质。 Supported by the National Natural Science Foundation of China (No.39600053) Corresponding author. Tel: 010-63291921; Fax: 010-63051130; E-mail: anwei@cpums.edu.cn 戴杰(首都
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