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文献和实验Replication timing by density transfer
, leu2-3,112, ura3-52, his6 in an A364A background. I aim for about 32 x 106 cells per time point. That gives enough DNA for at least 3 blots, leaving enough leeway for errors. 2. When the cell density is 2 x 106 cells/ml (OD660 ~ 0.16
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
7 mL of 1.15X ligation buffer. 19. Add 400 µL of 20% Triton X-100 (to a final concentration of 1%). Incubate with occasional gentle shaking for 1 h at 37°C. 20. Add 50 µL of T4 DNA ligase. Incubate for 4 h at 16°C
Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
of each axon, e.g., if it takes 20 seconds to collect a stack for an axon then at most you can sample 9 axons (9 stacks) if you are sampling every 180 seconds. The example shown in Figures 8 and 9 is a good representative result. About 10% of experiments
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