赛默飞世尔Thermo Fisher X50 BOTTLES

Clonality - X Chromosome Inactivation Assay

sample under study is ideal. D: PCR Set up the following PCR reaction: Cycles are as follows: E: Gel Electrophoresis Prepare gel consisting of 6% acrylamide as in LOH protocol. Add 4 µl dye to 20 µl PCR product. Denature the samples

Transformation of E. coli by Electroporation

for electroporation. 1. Use a fresh colony of DH5α (or other appropriate host strain) to inoculate 5 ml of SOB (without magnesium) medium in a 50 ml sterile conical tube. Grow cells with vigorous aeration overnight at 37EC. 2. Dilute 2.5 ml of cells into 250

Sample Preparation for Microinjection

minutes at 4°C (10,000 x g). Chill supernatant or load directly into capillary. Only centrifuge and use each aliquot once. Dyes, fluorescent injection markers [1] Successful fluorescent injection markers used to identify and follow injected cells