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- 2025年07月12日
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- 技术资料
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999
- 供应商：
北京泽平
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热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_7013130-16
中文名称：
英文名称：FERRIC CHLORIDE 100% W/V,500ML
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2
Preparation of End-Labeled DNA Probes by Conventional Kinase for DNA Footprinting Analysis
of approximately 500 μg/ml. 11. Store at -20°C. C. Kinasing Reaction 1. Combine the following: 20 μl of CIP-treated DNA (10 μg, from Step #B11) 4 μl of 10X Forward Kinase Buffer 14 μl of ddH2 O
7b. Pool the supernatants from step 6 into 500 ml bottles and add DNase-free RNase A and RNase T1 such that the final concentration of RNase A is 40 ug/ml and RNase T1 is 40 U/ml. Incubate in a 37degC water bath for 30 minutes. 8b. Add an equal
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