赛默飞世尔Thermo Fisher X50 BOTTLES
,ENVIRONMENTAL,SAMPLE BULK-PACKED,SINGL E USE,NARROW NECK HDPE 1000ML (33-2089-0032) 货号：T_70310294351- ￥70 - 1400
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- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70310294351
中文名称：
英文名称：X50 BOTTLES,ENVIRONMENTAL,SAMPLE BULK-PACKED,SINGL E USE,NARROW NECK HDPE 1000ML (33-2089-0032)
货期：热销品现货，其他请咨询
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文献和实验Transformation of E. coli by Electroporation
for electroporation. 1. Use a fresh colony of DH5α (or other appropriate host strain) to inoculate 5 ml of SOB (without magnesium) medium in a 50 ml sterile conical tube. Grow cells with vigorous aeration overnight at 37EC. 2. Dilute 2.5 ml of cells into 250
Cool growth competent E . coli
2O; add 10 ml 250 mM KCl; pH to 7.0 with NaOH Adjust vol to 1 liter; Autoclave. Just before use, add 5 ml sterile 2M MgCl2 per liter TB per liter 10 mM PIPES Na salt 3.35 g 15 mM CaCl2 (Fisher) 2.2 g 250 mM KCl (Aldrich) 18.64g 55
Fibroblast Cell Systems Instructions for Use
/EDTA Solution (T/E) (CC-5012) 1 x 100 ml bottle Trypsin Neutralizing Solution (TNS) (CC-5002) 1 x 100 ml bottle NOTE: If you use a different Clonetics® medium, see Appendix A. Product Applications Clonetics® Normal Human NHDF and NHLF
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