- ¥1207.90 - 3862.90
- 阿拉丁
- 上海
- R128623
- 2026年05月08日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
2-8°C储存；低温运输
- 英文名：
Ribonuclease B from bovine pancreas
- 库存：
现货
- 供应商：
上海阿拉丁生化科技股份有限公司
- 规格：
R128623-100mg/R128623-25mg
| 规格： | R128623-100mg | 产品价格： | ¥3862.90 |
|---|---|---|---|
| 规格： | R128623-25mg | 产品价格： | ¥1207.90 |
基本描述
产品名称：核糖核酸酶 B 来源于牛胰腺
规格或纯度：EnzymoPure™, ≥1,000 units/mg dry weight
产品介绍：
Pancreatic ribonuclease (RNase I) catalyzes cleavage of the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide forming a 2',3'-cyclic phosphate which may then be hydrolyzed to the corresponding 3'-nucleoside phosphate. Ribonuclease A has a molecular weight of 13,700 daltons. It operates in an optimum pH range of 7.0-7.5. Ribonuclease B has a molecular weight of 14,700 ± 300 daltons. It is a glycoprotein which possesses an amino acid composition indistinguishable from that of RNase A. It contains 6 residues of mannose and 2 residues of N-acetylglucosamine per molecule. It is a glycosylated derivative of RNase A. Ribonuclease is inhibited by heavy metal ions and it is competitively inhibited by DNA. Since Molecular Biology Grade RNase A (Product R128619) is essentially free of DNase and protease activities, this product is useful in removing RNA from DNA in nucleic acid work and where other enzymes are used or where intact proteins must be recovered.
级别：EnzymoPure™
产品规格参数
浓度：≥1,000 units/mg dry weight
储存条件：2-8°C储存
运输条件：低温运输
CAS编号和信息：9001-99-4
酶学委员会编号：I.U.B.: 3.1.27.5
单位定义：One Kalnitsky Unit causes an increase in absorbance of 1.0 at 260nm at 37°C and pH 5.0 when yeast ribosomal RNA is hydrolyzed to acid soluble oliognucleotides. One Kunitz unit equals 50 Worthington units.
功能和特点
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文献和实验How to Make Simple Solutions and Dilutions
= 200 ul = 0.2 ml C2 = the new concentration = 25 mg/ ml By algebraic rearrangement: V1 = (V2 x C2) / C1 V1 = (0.2 ml x 25 mg/ml) / 100 mg/ml and after cancelling the units, V1 = 0.05 ml, or 50 ul
High Molecular Weight Yeast Liquid DNA Preparation
Purpose: To isolate intact, high molecular weight DNA from yeast cells for subcloning and rare cutting restriction enzyme analysis. One can expect a yield of 100-200 � of DNA per prep. Time required: Day
), GSK-3b (dilution1:1,000; 1 h at RT), or b-actin (dilution 1:10,000; 1 h at RT). 5. Wash the membrane three times for 5 min each in TBS-T. 6. Incubate the membrane for 1 h at RT with 30 mL of TBS-Tcontaining anti-mouse or anti-rabbit IgG
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