- ￥699.90 - 2248.90
- 阿拉丁
- 上海
- R128619
- 2026年04月21日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C储存
- 英文名：
Ribonuclease A from bovine pancreas(DNase & Protease Free)
- 库存：
现货
- 供应商：
上海阿拉丁生化科技股份有限公司
- 规格：
R128619-5mg/R128619-1mg
| 规格： | R128619-5mg | 产品价格： | ￥2248.9 |
|---|---|---|---|
| 规格： | R128619-1mg | 产品价格： | ￥699.9 |
Pancreatic ribonuclease (RNase I) catalyzes cleavage of the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide forming a 2',3'-cyclic phosphate which may then be hydrolyzed to the corresponding 3'-nucleoside phosphate. Ribonuclease A has a molecular weight of 13,700 daltons. It operates in an optimum pH range of 7.0-7.5. Ribonuclease B has a molecular weight of 14,700 ± 300 daltons. It is a glycoprotein which possesses an amino acid composition indistinguishable from that of RNase A. It contains 6 residues of mannose and 2 residues of N-acetylglucosamine per molecule. It is a glycosylated derivative of RNase A. Ribonuclease is inhibited by heavy metal ions and it is competitively inhibited by DNA. Since Molecular Biology Grade RNase A (Product R128619) is essentially free of DNase and protease activities, this product is useful in removing RNA from DNA in nucleic acid work and where other enzymes are used or where intact proteins must be recovered.Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.
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文献和实验Clonality - X Chromosome Inactivation Assay
can utilize X chromosome inactivation (methylation) to determine the clonality status of a tumor or premalignant lesion in females. The technique is based on a methylation-sensitive restriction enzyme and analysis of a polymorphic locus on the X chromosome
DNase I Treatment Of Total RNA
Reagents1 U/ul DNase I from Epicentre Technologies10X DNase I buffer （200 mM Tris, pH 8.4, 20 mM MgCl2, 500 mM KCl）For 1ml:200 mM Tris, pH 8.4200 ul 1M Tris, pH 8.4500 ul 1M KCl20 ul 1 M MgCl2280 ul DEPC water25 mM EDTARNeasy Kit
组DNA等可能的DNA污染;体外T7, T3, SP6等RNA Polymerases催化的RNA转录后去除DNA模板; DNase I footprinting研究DNA-蛋白质相互作用;缺口平移(nick translatioin);产生DNA随机片断文库;细胞凋亡TUNEL检测中部分剪切基因组DNA作为阳性对照。 来源：从牛胰腺纯化得到。 分子量：约32kDa(单体)。 活性定义：37℃10分钟内，将能够完全降解1μg pBR322质粒DNA所需的酶量定义
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