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- 2025年07月15日
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- 克隆性：
EM92
- 标记物：
Purified
- 规格：
25ug
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文献和实验25 In Organello Footprinting of mtDNA
or intensify purine reactivity to DMS relative to naked DNA. The ability of DMS to readily permeate mitochondrial membranes permits detection of protein-mtDNA interactions that occur within purified organelles. We have employed two strategies (Southern
Gel-purified, agarased YACs for yeast transformation
Gel-purified, agarased YACs for yeast transformation Protocol from Andreas Gnirke (formerly of the Olson lab), with modifications by Anja van Brabant (Fangman/Brewer lab) Materials: 1. Yeast DNA plugs at 4 X
Intracellular Cytokine Staining Protocol
)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble) 5hrMonensinJES6-5H4IL-4mouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble) 5hrMonensinBVD6-24G2IL-6mouse
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