- ￥500 - 1230
- Elabscience
- 武汉
- AN010010P
- 2026年03月11日
- FCM
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
Mouse IgG1, κ
- 形态：
液体
- 保存条件：
Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12个月
- 级别：
科研级别
- 库存：
99
- 供应商：
Elabscience
- 宿主：
Mouse
- 应用范围：
FCM
- 靶点：
Oct-3/4
- 抗体英文名：
Purified Anti-Human Oct-3/4 Antibody[40/Oct-3]
- 抗体名：
Oct-3/4抗体
- 规格：
100μg/25μg
| 规格： | 100μg | 产品价格： | ￥1230.0 |
|---|---|---|---|
| 规格： | 25μg | 产品价格： | ￥500.0 |
Purified Anti-Human Oct-3/4 Antibody[40/Oct-3]
| Alternate Names |
OCT-3/4, Octamer-4, POU Class 5 Homoeobox 1, POU Domain Class 5 Transcription Factor 1, POU5F, OTF, POU5F1, OTF-3, OTF3, OTF4, Oct-3, Oct-4
|
| Cellular Localization |
Cytoplasm, Nucleus
|
| Clone No |
40/Oct-3
|
| Isotype |
Mouse IgG1, κ
|
| Verified Samples | Verified Samples in FCM: NCCIT cells |
| Dilution |
FCM 2 μg/mL(0.5×106-1×106 cells)
|
| Background |
Development of a multicellular organism from a single fertilized cell is regulated by the coordinated activity of DNA transcription factors. Oct3/4, a member of the POU family of transcription factors, functions in pluripotent cells of early embryonic stem cell (ES) lines and embryonal carcinomas (EC). Other members of the POU family include Oct1, Oct2, Pit-1, and unc-86. The POU domain, a 150-amino acid region that determines binding specificity, is conserved among these proteins and consists of 3 subdomains: POU-specific A and B subdomains and a homeobox-like subdomain. Oct3/4 is expressed in undifferentiated cells, but is lost as cells are induced to differentiate. Oct3/4 is not expressed in adult tissues. The interaction of Oct3/4 with SOX2, another embryonic transcription factor, produces an active complex that regulates expression of genes such as Nanog, UTF1, and FGF4. Although Oct3/4 is specifically phosphorylated on serine residues, this modification is not required for DNA binding, but may affect its transactivation potential. Thus, Oct3/4 is a transcription factor that plays an important role in determining early steps of embryogenesis and differentiation.
|
| Immunogen | Recombinant Human Oct-3/4 protein |
| Abbre |
Oct-3/4
|
| Swissprot |
Q01860
|
| Host |
Mouse
|
| Reactivity |
Human
|
| Clonality |
Monoclonal
|
| Purification Method |
>98%, Protein A/G purified
|
| Applications |
FCM
|
| Research Areas | Epigenetics and Nuclear Signaling;Developmental Biology;Stem Cells;Pluripotency Maintenance and Differentiation;Embryonic Stem Cells;Germline Stem Cells Markers |
| Tissue Specificity |
Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
|
| Concentration |
≥ 1 mg/mL
|
| Conjugation |
Unconjugated
|
| Storage Buffer |
Phosphate-buffered solution, pH 7.2, containing 0.05% non-protein stabilizer. Dialyze to completely remove the stabilizer prior to labeling.
|
| Storage |
Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles.
|
| Expiration Date |
12个月
|
| Shipping |
Ice bag
|
Purified Anti-Human Oct-3/4 Antibody[40/Oct-3]Elabscience®流式抗体致力于为您的流式细胞分析实验带来更优质的体验，已有3,000+指标，选用经典克隆株纯化抗体，产品涵盖抗人/大鼠/小鼠等，指标丰富。
Purified Anti-Human Oct-3/4 Antibody[40/Oct-3] 4°C 保存，保质期 12 个月；或于 -20°C 保存（适合长期保存），避免反复冷冻/解冻。
Purified Anti-Human Oct-3/4 Antibody[40/Oct-3]产品更多产品信息查看欢迎咨询我们和搜索武汉伊莱瑞特Elabscience官网查看。
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文献和实验Isolation of a Purified Epithelial Cell Population from Human Colon
. For the human colon, isolation techniques to allow direct study have been described for mononuclear cells in the lamina propria, smooth muscle cells at or below the muscularis mucosae, and cells of the enteric nervous system, located between the subserosa
To fix, 1/10 volume of 37 formaldehyde is added to the culture, which is shaken for a further 40 minutes at room temperature. Cells are then recovered by centrifugation at 1400 X g for 2 minutes, and washed (i.e. gently resuspended then recovered
of 0.75 to 1. To fix, 1/10 volume of 37% formaldehyde is added to the culture, which is shaken for a further 40 minutes at room temperature. Cells are then recovered by centrifugation at 1400 X g for 2 minutes, and washed (i.e. gently resuspended
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