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- Beckman Coulter贝克曼库尔特
- 美国
- 366704
- 2025年07月14日
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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
the residual platelets. Remove supernatant, resuspend cells in 1X PBS, and repeat the wash once to remove remaining platelets. 4. Resuspend in 20-30 mL of 1X PBS and then count the cells. Aliquot 0.5 x 106 cells for fluorescence-activated
Synthesis of a 4‐Selenothymidine Phosphoramidite and Incorporation into Oligonucleotides
. and Huang, Z. 2004. Enzymatic synthesis of phosphoroselenoate DNA using thymidine 5′‐(a‐P‐seleno)triphosphate and DNA polymerase for X‐ray crystallography via MAD. J. Am. Chem. Soc. 126: 448‐449.
Differential cDNA Screening Procedures
2 min. 4.Incubate nitrocellulose discs in denaturation buffer 30 sec, then neutralisation buffer for 30 sec. Standard recipes. 5.Wash filters in 2 x SSPE, rubbing off any adhering agar (This doesn't affect plaque blots). 6.Air dry the filters
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