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- Beckman Coulter贝克曼库尔特
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- 2025年07月15日
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文献和实验j5 DNA Assembly Design Automation
to a DNA synthesis service provider, and designs hierarchical DNA assembly strategies to mitigate anticipated poor assembly junction sequence performance. Software integrated with j5 add significant value to the j5 design process through graphical user
The ribonuclease protection assay (RPA)
by gentle flicking and quick spin in a microfuge. Incubate at 37°C for 30 minutes. 3. Add the following reagents (in order) to each 1.5 ml Eppendorf tube: 26 µl 20 mM EDTA 25 µl Tris-saturated phenol 25 µl chloroform:isoamyl alcohol (50
GENERAL PROCEDUREThe most popular extraction procedure is that of Folch (Folch et al., J Biol Chem 1957, 226, 497):Folch method The tissue is homogenized with chloroform/methanol (2/1) to a final volume 20 times the volume of the tissue sample (1 g
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