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- Beckman Coulter贝克曼库尔特
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- 2025年07月16日
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文献和实验product is detected in the PCR process as a significant increase in fluorescence, and the lower the Ct value. Ct values are very reproducible in replicates because the threshold is picked to be in the exponential phase of the PCR, where there is a linear
RADIOLABELING OF PROBES FOR ELECTROPHORETIC MOBILITY SHIFT ASSAYS
of solution is in the tube. Check all components of the beta shield device to ensure that there is NO contamination. 14.Add 1μl of Lower strand oligonucleotide to the eppendorf tube,and flick gently to mix. Remove the 100℃ block from the heater and place
Radiolabeling of probes for electrophoretic mobility shift assays
Materials: g -32 P ATP (New England Nuclear/Perkin Elmer, CAT# NEG-035C) “Upper strand” oligonucleotide (at 0.5 ug/ul) “Lower strand” oliognucleotide (at 0.25 ug/ul) T4 polynucleotide kinase (New England Biolabs) 10X T4 polynucleotide
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